XB-CLONE-1724853
Clone Name: XL511o06ex
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Gene:
XB5729910.L
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Species: Xenopus laevis
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Sequence:
5' EST: BP704440 [+]
caaagattccctcccagcagcacaggtgggtcagtgtgctgcataaatgccttggccggagtccctaatctaatgctactctgcagtaaaataattttta
taccgtttgagtggattttgatctaatgacacttttatggagagcaccccttttgcacgagaaagaagaagccaaaaggcagtcaagactcttaacaaag
gtgaaaagttagttatgaagcatgttaatgtgccaagcattgataaccagtttgcagagattggcaacctctacaatgatcaggtggagcaccatgagac
catgacacagtgtatcatccgcctggcaaatatcagtgcctcgtctcctgacctcaccacctgtatacagaggctgaaagaaaactatcgtgactatgat
gttaaagttctgatggaagggtacaatttctctcttctggtgaaaacggaaaaggaatttccagaagacttaaaacaagcacaggagatcatgaaaaatt
tgagtcgagcatccaagcttctcatcagtaaccaaaccagactgggggagatgatgttcacagttctacagttgcaaaataaggttgatatgaagattag
cataactgaggccaacactgtttacttggaccaagtacgcctaaaagaaaacctggaagagaatctacaaaaaacagataaagccagacaactgtctaaa
gaatatgaagaagatgccaataaagttattagagaattagctaagatttctggagctgcattttgaaataatcaatgctctttactctctttatcaatgc
tctttacctggttttgcaagatatacagtaaa
3' EST: BP737254 [+]agttttcngctcgttcataatcagtaaaaaaacattactctgctcccattaaccgggttgaccattgcctacggcaatatatactgtagctgtgggaatg
tttattatgtctaattactttatgtgttctgaacacttttttagccaataattcaattaaaaatatacagacaagttcaaacaagaagataatctgtaca
taacgccagtaaaataaaaaaagttatataatattgtttgctattttctacagtgtaatcatgcatctgaggattctggtacaaaacataaaataatgtg
atgtactataccctaataacaccgaagctccatataaatgactttaatagcaccagcataatgcttaaattattattaggaaaactaaaaattcccatca
ggggctgcagctggctgtggatgtaggtcttgtagatgtaggttgatctgaacaatatgaataatgtgacataatatgattgtccctggaacaggattgt
aagatcagcttgatatgaccaactgtctggcataccttgtaaagccagattatgtacttgtaagcagtagagaccatcatggttatatgcatgggtaaaa
gataacttggttgcagacattattcaatattcaatcctgggccaatcttgtttaatttgtcatatttagtgctcataaatcagataccctatatcagagc
aaaatgtgatgcaaacncaaccttggaatttgtgatgaaacagttgattaaactagtttgcttcagtaaaaccagtggcattgcatctaccatttttgaa
caggctcncattttacagaaaaaatccctataaaa
Library:
Description: About 500 pieces of anterior neural plates were dissected from stage 12-12.5 embryos. The neuroectoderm layer was separated from the underlying mesendoderm layer in 1 x modified Barth's solution containing 1-2 mg/ml collagenase. After poly (A)+RNA selection by an oligo(dT) cellulose column, a cDNA library was made with the SuperScript plasmid system for cDNA synthesis and plasmid cloning (Invitrogen) and the pCS105 vector (Hsu D.R. et al., Mol Cell. 1, 673-83, 1998). The first strand cDNA was synthesized with oligo dT-NotI primer. After second strand synthesis, SalI adapter (gtcgacCCACGCGTCCG; the lower case letters show SalI site) were ligated and the cDNA was directionally cloned with SalI at the 5' end and NotI at the 3' end. The average insert size is about 2kb and the host bacteria is XL1 blue (Stratagene) (?).Vector Details | Vector Map |
Name: pCS105
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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