XB-CLONE-1715926
Clone Name: IMAGE:7297296
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Gene:
rlf.S
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Species: Xenopus laevis
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Sequence:
Full length sequence: BC133797 [+]
GCTGTATGGTGTGGTGTGTGTGTTGCCTACGCGCGGCAAAGGCGAGAGAAGATGGCGGACGGGAAGGGAGAGTCCCGAACGGGGCTAGCCAGCAACGACA
TATCGCCTTCTACGCTTTCCGCAGCGAGCACACACCGCTTGATAGATGACTTGAAAGAACTAAAGGACATGCTGAAAGACGACGATGTGTCTGCGCTCTC
CTCTTCTGTGTATTGCGGTCGCTTCTGCGAGATACTAATGCAACATACTGAAGAAAGTACAGCTTCAGAAAATGTAATTACTTGTGCAGGTGTTTATGGA
ACTGCTTTGCAGAGCTTTGCTACTGCTCGCCCATACCTCACTACTGAATGTGAAGATGTTTTGCTTCTACTCGGAAGGCTTATGTTGAGTTGCTTTGAAG
TAATACTTTCAATGACTGAAGATGACCTCTCGTGTGATTATGGATTGCAATTAAAGAAATCTATTGTGGAGTCACATAACATACTAGTAGAATTTGGAAA
TAACAATTTACAGTTATTGATTGATGTTGTCCAGAATGGAGGAGCTTGGAAAAATCATGTTCTAGTTAGAATTTTGTCTCAACAACCTGTTGAGCCAGAA
GAAGTCCAGAAATTGATTTCACAAGAGGGGTCCTGTTTTCTGCAGATGCGAATTAAGCACTTGATGAAATCAAACTGCATACAGCAAGCAATGATCTTAT
CAAAGATTTGTTCAGACTCGGCAGAAATGTCGAATGATTTCTTTTTTCGGCAGTCATTTATCACATGCCTATGTACTATGTTACCCAATGAGGAAGCTTT
TAAAGAGATTTCGAAGATGGATGGAAAGGAGGTATTGGATACAATATGTAATCTGGAATCTGAAGGACAAGATAACACAGCATTTATTCTCTGCACTACT
TATCTTACCCAACAACTGCAAAATGAAATTACTTCTTGCTCCTGGGAGCTAACCCTATTTTGGAGCAAGCTGCAGAGAAGGATTGATCCTGCTTTAAATA
CGTTTCTTGAAAGATGCAGACAGTTTGGTGTCATTGCTAAAACACAGCAACACCTTTTTTTTCTTATCAGAGTTGTGCATGCAGAGGCTGGAGACGAGGG
TATTCCAATTGCTATAGTTTTATGCATAAGAGCCCTTCAGATTCCATCAAATGAATCCGATGCAACAAAGACATCTGTTTGCAAAACAATAGCTTGCCTC
TTACCACAAGATTTGGAGGTTAGGCGAGCCTGCCAACTCACAGAATTTTTATGTGAGCCAACGTTTGATGGTCTAAATATATTGGAGGAGCTCTTTCAGC
AGCCTGATCAAAAAAATGATGAAGAATCAAGTGTTATTTCAAACTCCCTGCGCTGTGAACTTTTGCTTGCTTTAAAGAGTCACTGGATGTTTGATCCTGA
ATTCTGGGATTGGAAAACATTAAAGCGACATTGTCTTAAACTTCTAGGAAAAGAAGTATCTGATGAGGAGGAAGTGAATTTTGACAATCCTTTGGTTAAC
GAGACAGATGTCTTCAACCCCTCATTAGGTAGCTATGAGGACTTAGTCGAAAAAGCACAAGCATCTCCGGAATATGTTACTAACGAACTTGAAACTACTA
AAGTGAAAAAGCCAGTTGGGTCATCTGAAAGGTATAAAAGGTGGCTTCAATATAAATTTTACTGCTTAATCTGCAACAGAGAAGTAATTGAGGCCAGGAT
TCTCCATCATTCAAGGATGCACTTTGCTGATGGTATATACACTTGTCCTGTTTGTATTAAGAAATTCAAGAAGAAGGATATTTTTGTACCTCATGTAATG
GACCACATGAAAATGCCCATGCGGCATCGACCAAAAAAAAAAAAAAA
5' EST: CO389875 [+]ccatcttaggcgattgggtacgggcccccctcgagtttttttttttttttggtcgatgccgcatgggcattttcatgtggtccattacatgaggtacaaa
aatatccttcttcttgaatttcttaatacaaacaggacaagtgtatataccatcagcaaagtgcatccttgaatgatggagaatcctggcctcaattact
tctctgttgcagattaagcagtaaaatttatattgaagccaccttttatacctttcagatgacccaactggctttttcactttagtagtttcaagttcgt
tagtaacatattccggagatgcttgtgctttttcgactaagtcctcatagctacctaatgaggggttgaagacatctgtctcgttaaccaaaggattgtc
aaaattcacttcctcctcatcagatacttcttttcctagaagtttaagacaatgtcgctttaatgttttccaatcccagaattcaggatcaaacatccag
tgactctttaaagcaagcaaaagttcacagcgcagggagtttgaaataacacttgattcttcatcatttttttgatcaggctgctgaaagagctcctcca
atatatttagaccatcaaacgttggctcacataaaaattctgtgagttggcaggctcgcctaacctccaaatcttgtggtaagaggcaagctattgtttt
gcaaacagatgtctttgttgcatcggattcatttgatggaatctgaagggctcttatgcataaaactatagcaattggaataccctcgtctcc
3' EST: CO554246 [+]gctgtatggtgtggtgtgtgtgttgcctacgcgcggcaaaggcgagagaagatggcggacgggaagggagagtcccgaacggggctagccagcaacgaca
tatcgccttctacgctttccgcagcgagcacacaccgcttgatagatgacttgaaagaactaaaggacatgctgaaagacgacgatgtgtctgcgctctc
ctcttctgtgtattgcggtcgcttctgcgagatactaatgcaacatactgaagaaagtacagcttcagaaaatgtaattacttgtgcaggtgtttatgga
actgctttgcagagctttgctactgctcgcccatacctcactactgaatgtgaagatgttttgcttctactcggaaggcttatgttgagttgctttgaag
taatactttcaatgactgaagatgacctctcgtgtgattatggattgcaattaaagaaatctattgtggagtcacataacatactagtagaatttggaaa
taacaatttacgttattgattgatgttgtccgaatggaggacttggaaaatctgttctagtagaatttgtctcacactgtgagccaaaagtcagaatgtt
cccagagggtctgttctggatgcatagcctgtgatcactgctccagagttatcaatgtcacggatgcagatcttcgccttcgctgctgtctggctaatca
gggaggtgattgagactctccacggggntgagagggtaaacacacaaaaagaagtaatnt
Library:
Description: Library was prepared from 50 ug of total RNA by oligo-dT priming and AMV reverse transcriptase. After addition of EcoRI linkers and EcoRI-XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-4B columns and fractions containing cDNAs larger than 500 bp were ligated into EcoRI-XhoI-digested lambda ZAPII (UniZAP-XR) and packaged in vitro. Average insert size is 1.4 kb. The original library contained 6 x 106 recombinants, of which 3 x 106 were amplified and stored at 70C in SM buffer containing 7% DMSO. 3 x 106 pfu were mass excised and the resulting phagemids used to infect Top10F'. References: Science 253, 196-196 and Methods in Molecular Biology 97, 555-574. Additional sequences from this library have been deposited under the name Xenopus laevis dorsal blastopore lip. Library constructed by Bruce Blumberg (University of California, Irvine, Department of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pBluescript SK-
Type: phagemid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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