XB-CLONE-1702323
Clone Name: XL444h11ex
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Gene:
rrm2.L
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Species: Xenopus laevis
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Sequence:
5' EST: BP683937
[+]
ccctaacgtaaactccgacaggatctcccggggaacacagaaagaactggtctgtcagagcgtaaaggatcctctgatacaggatgagcctctcctgagg
gacaacccaggccgctttgtgatcctgcctattgagtatcatgacatatggcagatgtacaagaaggcagaggcttctttctggacggcagaagaggtgg
atctttctaaagatcttcagcactgggaagctttaaagcctgaagaaagatacttcatttcttatgtgttggccttttttgcagcaagcgatgggattgt
taatgagaatctggtggagcgcttcagccaggaagtgcaagtaaccgaggttcgctgcttttatggttttcaaattgcaatggagaacattcattctgaa
atgtacagtcttctgattgacacatatattaaggatcccaaggaaagggagttcctcttcaatgcaatagaaaccttgccctgtgttaaaaagaaggcag
aatggtccctgcgttggataagtgaccgagaagcaccttattgtgagcgccttgtggcctttgctgctgttgaagggatatttttctctggttcatttgc
ttccatattctggctgaagaaacgagggctgatgcctggactcactttctctaacgaacttattagcagagatgaaggtttgcacactgactttgcttgc
cttttgtttaaacatgttgtacacaaaccctcagagcaacgtgtcagggaaattatcattgatgcggtcaagattgagcaggaattcttgactgaagctc
tacctgtggagctaaattggtatgaactgcacactgatg
3' EST: BP719953
[+]ttcttaaataaaactctaacaatcaaatacctagcaaactgcagaatgtttttcacttcaacacaatgcactgcccagcatatacaagcttccttacaat
cagagcaggtatcagcgcaagatttgtttatgtatttgcttgcttttaacagggccagcaaaggcatgtacaagtaaccatgggatcgcaggctagaaca
gactaaaacaaaaatgtatcagtgcagtgcaaatcaatgcacaaatgtaagatatttaaaagtcagcatttagagtgaattgattttccgtggccttggc
catgacacccatcttctggtattcaccaactttcttctcaaagaaattggtctttccttctaaggagatgttttccatgaagtcaaatggattctcagac
ttaaaaatctttttaaaaccaagctccagcagaagccgatctgctacaaattcaatatactgcttcatcagtgtgcagttcataccaattagctccacag
gtagagcttcagtcaagaattcctgctcaatcttgaccgcatcaatgataatttccctgacacgttgctctgagggtttgtgtacaacatgtttaaacaa
aaggcaagcaaagtcagtgtgcaaaccttcatctctgctaataagttcgttagagaaagtgagtccaggcatcagccctcgtttcttcagccagaatatg
gaagcaaatgaaccagagaaaaatatcccttcaacagcagcaaaggccacaaggcgctcacaataaggtgcttctcggtcacttatccaacgcagggacc
attctgccttctttttaacacagggcaaggtttctattgcattgaa
Library:
Description: About 500 pieces of anterior neural plates were dissected from stage 12-12.5 embryos. The neuroectoderm layer was separated from the underlying mesendoderm layer in 1 x modified Barth's solution containing 1-2 mg/ml collagenase. After poly (A)+RNA selection by an oligo(dT) cellulose column, a cDNA library was made with the SuperScript plasmid system for cDNA synthesis and plasmid cloning (Invitrogen) and the pCS105 vector (Hsu D.R. et al., Mol Cell. 1, 673-83, 1998). The first strand cDNA was synthesized with oligo dT-NotI primer. After second strand synthesis, SalI adapter (gtcgacCCACGCGTCCG; the lower case letters show SalI site) were ligated and the cDNA was directionally cloned with SalI at the 5' end and NotI at the 3' end. The average insert size is about 2kb and the host bacteria is XL1 blue (Stratagene) (?).Vector Details | Vector Map |
Name: pCS105
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40545.jpg) |
Usage:
Suppliers: