XB-CLONE-14578549
Clone Name: sox1-HAR321
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Gene:
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Species: Xenopus tropicalis
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Description: EXRC Clone Number HAR-321. Xenopus tropicalis SRY (sex determining region Y)-box 1. The Sox1 coding region was excised from plasmid #320 with Sal1 and Not 1. This clone of Sox1 has removed a lot of the 5' and 3' UTRs. To generate RNA for injection.
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Sequence:
Full length sequence: Sox1 truncated UTRs-1_FullLength [+]
TCAGGCCTTGCGGCCGCTCTAGAAAGAGCAACAAATCAGGGTTGTCTGTTTGTTTTACTGTATTTTTAGCACGGATCTGAGACTTCAATTTCTTACAAGA
ACATCAAAACATCTCTGTACAAAACAAAAAGAGAGGGCAGTTCTGGGAAAGTGGAGGGAAGCGTGCTGCCCTGGTCCTTGATGAGCAGGCAGATTGCATT
GTTCCTAAAAGGGTGAGCACAGTCCCCTACCATCACTTATCCGTGTCATTATCACTGTCCTGGTTCAGAAAGTCTGTCAGATGTGTGTGAGGGGCACGGT
GCCGGTTACCCCCGTCCCTGCCCCCTGGTAGTGCTGGGGCAAGCTGTGAAGTCTACTAGTTGCTGCTGCAGCCGCCGCTGCTGCCGCTGCCGCAGCAGCG
GCCGGGTCCCCGGCTTCCCCCCCACTAGGCAAGTACATGCTGATCATCTCCCGTAGATCTCCGGGACAGGGCGGCCGGTTATGGGGCCCACCTCCGCTCA
CTGGGGGACTGACACTGGGTTCGGATTTCACCAGGGATCCCAGTGCCCCTAGGGCACCAGATGAAGCTGCAGCGGCGGCTGCAGCAGCTGCAGCCGCCGC
CACCGCGGCCGAGGACGGAGAACCCTGGTGCTGCTGTTGGCTGGAGCTGTAGCCCAGCGCCCCATAGCCAGAGGGAGAAGCGGTCATGTAGGTCTGGGCA
CCGGGCAGCGGGCTGTACTGCAGGGCGCTCATGTCATAACGGTGCATGGGTTGGGGCGGAGGGGGTGGCGGGTGTGGCGTANGGTTGTGGTGCGGATGGT
GATGCGGGTGATGGTGTGGGGTGGGTGGGGG
Construction:
Insert:
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Size: | Source Tissue: | Stage: |
Vector:Vector Details | Vector Map |
Name: pCS108
Type: plasmid
5' Restriction Site: null
3' Restriction Site: null | |
Usage:
RNA injection:
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Probes:
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Type |
Size |
Promoter |
Linearization Site |
Description |
sense |
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SP6 |
AscI |
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Suppliers:
Search for clone at:
EXRC