XB-CLONE-14575382
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Clone Name: tbx5-451
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Gene:
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Species: Xenopus laevis
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Description: EXRC Clone Number 451.
Xenopus laevis T-box 5.
Accession Number: AB032944. PCR fragment of xenopus tbx5 cDNA.
To generate probe for in situ and mRNA for injection.
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Sequence:
Full length sequence: tbx5_FullLength
[+]
NNNNNNNNNNNNNNNTTGGNANNTCTCCCNTATGGTCGACCTGCAGGCGGCCGCACTAGTGATTCGGGATCCGACGATCACCGTTACAAGTTCGCTGATA
ACAAATGGTCAGTCACAGGCAAGGCTGAACCCGCTATGCCAGGGCGTTTGTATGTTCACCCAGATTCACCAGCCACGGGCACACATTGGATGAGGCAGCT
AGTATCCTTCCAGAAGCTGAAGCTCACAAACAACCATCTGGATCCATTTGGACATATCATCCTGAATTCAATGCACAAATACCAGCCACGGCTCCATATT
GTTAAGGCAGACGAGAACAATGGCTTTGGGTCTAAGAATACGGCTTTCTGCACTCATGTATTTTCGGAGACTGCTTTTATTGCTGTCACCTCATATCAGA
ACCACAAGATCACGCAGCTAAAGATAGAGAACAACCCATTTGCCAAAGGTTTTCGGGGGAGTGATGACATGGAGCTACACCGGATGTCCAGGATGCAAAG
CAAAGAATACCCAGTGAATTCCAATCCCGCGGCCATGGCGGCCGGGAGCATGCGACGTCGGGCCCAATTCGCCCTATAGTGAGTCGTATTACAATTCACT
GGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAA
GAGGCCCGCACCGATCGCCCTTCCCAACAGNTGCGCAGCCTGAATGGCGAATGGACGCGCCCTGNNNCGGCGCATTAAGCGCGGCGGGTGTGGTGGNNAC
NCGCAGCGNGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTTCNTTTCTCGCCNCGTTCGCCGGCTTTCCCCGTCAAGC
TNTAAATCGGGGGGCNCCCCTTAGGGTCCCNANTTANTGTTNNCNGNCCCNCNCCCCAAAAAACTTGATTAGGGNGANGGTTCNCGTNNGNNCATCTCNT
NTATAGACNGGTTTTTCNTCTTTTGNCNTTGNATTCACGTCCTTTATTNNNGNANNNTTGNTNNNAACTGGNACAACNNTNACTCTANNNNGGTTATCCT
TNATNTNTNNNNGATTNGCCCATTTTGTCTATTGGNNTNNANAATGANCTNNNNTANNNNTNNTANTGTTNANNNNNCCANNNNNTNANNNNNACNNNTC
NCTNNTGNTGTTATTTTCCNNNTNNTNTTNTNNNGCGTATGTCNTANCCNNNNNNNTGTGNNNNCTTTNCNGNNAANTNNNGTNCNNNACNNCTNTTTNT
NNNNN
Construction:
Insert:
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| Size: 399 bp | Source Tissue: | Stage: |
Vector:| Vector Details | Vector Map |
Name: pGEM-T
Type: plasmid
5' Restriction Site: null
3' Restriction Site: null |  |
Usage:
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RNA injection:
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Probes:
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| Type |
Size |
Promoter |
Linearization Site |
Description |
| sense |
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SP6 |
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Linearize with NcoI. |
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Whole mount in situ hybridization:
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Probes:
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| Type |
Size |
Promoter |
Linearization Site |
Description |
| antisense |
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T7 |
NotI |
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Suppliers:
Search for clone at:
EXRC
