XB-CLONE-12442424
Clone Name: xa-1-KL44
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Gene:
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Species: Xenopus laevis
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Description: EXRC Clone Number KL-44.
Xenopus laevis anterior and ectodermic-specific protein
full length XA-1.
To generate probe for in situ and mRNA for injection.
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Sequence:
Full length sequence: pXA-1_FullLength [+]
NNNNNNNNNNNNNNNNNNNTNNGCGGNNNNNNNNNNNNNNNNNNNTNNNNNNNTTTGANNAGAGATANACGGGATTTCCAGCCATGTTTTTCTACGTTCT
TCTGCTTGCTCTAATGGCCCAGGGCTGGAGCCTTCCACAAGGGAAGACAGGAGAAGATTCTCCAGTCTTCAGGCCTCCTTCTCCACCCATGGGCCCTAGC
CTTCCTCCACCTGTCAGCCATGACCTGCACAGGCCATCGGGTCACCCTGAGGAGTTCAGAACTGGAGCATCCTTACCTCCAAAAGAAACCCCCAATGAAC
CGAGGCATGGCAGGCCTAAGAGAGATCTTCATCATGGGAAAGTTGTCCCCACTGGAGTCCCTCATCACACAGGAGAAGTCCTACATCACACAGACTGTTC
TTCTAATACCCACAAGTCACATGAAGAAAACAGACCTAAAGGGTTCAGGACTGGACGTCCCTTGCTACCCATAAAACCTGAGCATGGCAGGCACAGGAGA
GATCTTCATCATGGGAAAGCTGTCCCTACTGGAGTCCCTCATCACACAGAAAAATTCCACAACGGCAGCAATGGAAAAAGCCATCCTCCCCGACCAGGCC
ATTCCACCTCTGCCCACAATGACAACTCAAGTGAAGAAAAGAGACCAAAACATGGTCAGGAACAAGGAAAGAAGCATTAGTAAACATAAAAAGAAGAAAA
TTAAAGAGGAAACCGAGTACATTGGATGGAATTATTAATAAGTTATGGCAAAACCAATAAAGTGATAATGANNNAAAAAANNNAAAANANAAAAAAAAAA
NAAAAAAAAANNNNAAATNAANATAANNANATANNAANANAACAAAANAGGGANCNGGANTTCNNTATCNGTTTATCNATCCCNNCNNCCTNNNGGGGGG
GGCCCCGGTACCCANTTCCCCCNANAGGGNGNCNNATTACAATTTNNNNGGCCNNCCTTTTNNNANCGCCNTGCCGGGGAAAACCCCTGGGTTTTCCCCA
ATTATANCCCCCTTGGANAACTNCCCCCTTTTCCCCNNNNGGGGTAAAAACNAAAAGNNCCCNNCCCANNTCGCCCNTCCNAANNNTNGNCNAACCCTGA
NATGGNNAAAGGGAAAANTNGNAAGNNNNNNANNATTTNTNGNNAAAANNNNNNGNTNAAANTTTTNGNTAAANNCNNNCTCNTTTTTTTANACCAAAAG
GNCNNNAAATTCGGCAAAANNCCCTTTNNAAATTCAAAAAAANTAANACCGAANATATGGGGTTNAANNNTTTNTTCCCNAGTTTNNAAANACAGNAGTN
NCCCNTTTTTTAAAANAAANGGTGGNNNNNNNNANCNGTNNAAAGGGNGGNAAAAANCCNNTNTATTTNNGGGGGNNNNNGGNCCCCNNNNNNCNNNNNA
NNNNNNNCTACCCCNNNTANNNNNGNTTTTTTTTGGGGGGNCNCNNNNGGGNNNCCNNNAAAANNNNNNNNNAAANNNNNNNNNANNNCCCNANNANNGG
NNNACCCCNNNNNAATNTTTNNNNAANNTTTNNNNNNNNNNGNNNANNANNNNNNNNNNNNNNNNNANNNNNNNNNNNNNN
Construction:
Insert:
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Size: 800 bp | Source Tissue: | Stage: |
Vector:Vector Details | Vector Map |
Name: pBluescript II SK+
Type: phagemid
5' Restriction Site: NotI
3' Restriction Site: EcoRI | |
Usage:
RNA injection:
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Probes:
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Type |
Size |
Promoter |
Linearization Site |
Description |
sense |
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T3 |
EcoRI |
HindIII or XhoI can also be used |
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Whole mount in situ hybridization:
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Expressed during stages 12 to 48 in cement gland and head ectoderm.
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Probes:
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Type |
Size |
Promoter |
Linearization Site |
Description |
antisense |
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T7 |
NotI |
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Suppliers:
Search for clone at:
EXRC