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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
fga
|
|
laevis
|
NF stage 37 and 38
|
endoderm
,
midgut
,
hindgut
,
alimentary system
,
foregut
,
[+]
|
foxa2
|
|
laevis
|
NF stage 18
|
endoderm
,
ventral
,
anterior neural fold
,
anterior
|
pdx1
|
|
laevis
|
NF stage 37 and 38
|
endoderm
,
alimentary system
,
stomach
,
pancreas
,
foregut
,
[+]
|
nr1h5
|
|
laevis
|
NF stage 37 and 38
|
endoderm
,
alimentary system
,
liver
,
foregut
|
hhex
|
|
laevis
|
NF stage 13
|
endoderm
,
dorsal
,
anterior
|
hhex
|
|
laevis
|
NF stage 24
|
endoderm
,
foregut
,
liver primordium
|
hhex
|
|
laevis
|
NF stage 32
|
endoderm
,
alimentary system
,
liver
,
pancreas
,
foregut
|
hhex
|
|
laevis
|
NF stage 42
|
alimentary system
,
liver
,
pancreas
,
foregut
|
|
|
Fig. 5. Liver and ventral pancreas defects in hhex morphants. Probes or methods for whole mount staining are indicated on the left. (A and B) Bisected embryos, dorsal toward top. (C) Lateral view, head toward the left. (I and J) Bisected embryos, anterior toward the left. (K) Lateral view, head toward the left. The white arrowhead (N) indicates the missing expression of fibrinogen in the liver anlage. (S) Bisected embryos, anterior toward the left. Phenotype is as follows: B, 19/20; D, 20/22; F, 24/25; H, 34/41; J, 25/27; L, 38/44; N, 19/20; P, 36/42; R, 16/72 (for detailed phenotype analysis, see Fig. S7); T, 22/23; V, 41/41. (W) Statistical analysis of phospho-histone H3-positive cells in the outlined areas in U and V. Thirty-four samples from 17 bisected embryos were counted from either control MO or hhex MO-injected embryos. P value (0.309) was determined by the paired, two-tailed t test. |
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Fig. S7. Knockdown of hhex led to specific loss of Xptf1a/p48 expression in ventral pancreatic buds. (A, C, E, and G) Shown are the lateral view, head toward the left. B, D, F, and H are group photos of embryos represented in A, C, E, and G, respectively. Numbers of embryos showing phenotypes illustrated in C, E, and G are given in the individual images. |