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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
foxf1
|
|
laevis
|
NF stage 33 and 34
to
NF stage 35 and 36
|
head region
,
pharyngeal arch
,
lateral plate mesoderm
,
anterior dorsal lateral plate region
,
olfactory region
|
sox2
|
|
laevis
|
NF stage 33 and 34
to
NF stage 35 and 36
|
otic vesicle
,
brain
,
spinal cord
,
endoderm
,
pharyngeal arch
,
[+]
|
nkx2-1
|
|
laevis
|
NF stage 33 and 34
to
NF stage 35 and 36
|
brain
,
forebrain
,
endoderm
,
trachea
,
lung
,
[+]
|
shh
|
|
laevis
|
NF stage 33 and 34
to
NF stage 35 and 36
|
endoderm
,
pharyngeal arch
,
mandibular arch
,
hyoid arch
,
branchial arch
,
[+]
|
wnt2b
|
|
laevis
|
NF stage 33 and 34
to
NF stage 35 and 36
|
otic vesicle
,
pharyngeal arch
,
pineal gland
,
lens placode
,
lateral plate mesoderm
|
|
|
Fig. 3. Osr1/Osr2 are required for wnt2b and raldh2 expression in the lpm. (A-X) Analysis of stage 34/35 unilateral-injected embryos shows that Osr1/Osr2 are required for expression of nkx2.1 (A-C) and sox2 (D-F) in the epithelium, whereas shh (G-I) is unaffected. In the lpm, wnt2b (J-L) and raldh2 (M-O) are absent, while foxf1 is unaffected (P-R). Black arrows indicate normal expression on the control side and red arrows indicate lack of expression on the Osr1/Osr2-MO-injected side (right). Endoderm outlined by a yellow line. Numbers of embryos with the shown phenotype are indicated. fg, foregut; hg, hindgut; de, dorsal endoderm; pe, posterior endoderm; e, esophagus; l/t, presumptive lung and trachea; lv, liver; lpm, lateral plate mesoderm; k, kidney; s, stomach. |
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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
osr2
|
|
laevis
|
NF stage 33 and 34
to
NF stage 35 and 36
|
head region
,
pronephric duct
,
endoderm
,
foregut
,
lateral plate mesoderm
,
[+]
|
sox2
|
|
laevis
|
NF stage 33 and 34
to
NF stage 35 and 36
|
otic vesicle
,
brain
,
spinal cord
,
endoderm
,
pharyngeal arch
,
[+]
|
nkx2-1
|
|
laevis
|
NF stage 28
to
NF stage 32
|
brain
,
forebrain
,
midbrain
,
hindbrain
,
endoderm
,
[+]
|
nkx2-1
|
|
laevis
|
NF stage 35 and 36
|
brain
,
endoderm
,
eye
,
thyroid primordium
,
lung primordium
|
wnt2b
|
|
laevis
|
NF stage 33 and 34
to
NF stage 35 and 36
|
otic vesicle
,
pharyngeal arch
,
pineal gland
,
lens placode
,
lateral plate mesoderm
|
oxsr1
|
|
laevis
|
NF stage 33 and 34
to
NF stage 35 and 36
|
brain
,
pronephric duct
,
endoderm
,
eye
,
foregut
,
[+]
|
|
|
Fig. 4. FGF, Wnt/β-catenin and RA signaling regulate Xenopus lung development in a pathway with Osr1/Osr2. (A-D) Embryos were treated from stages 25-35 with DMSO vehicle (A), 100 μM PD173074 (B), 50 μM XAV939 (C) or 10 μM BMS493 (D), and assayed at stage 35 with the indicated probes. (E). Embryos were treated between stages 28 and 35 with vehicle (DMSO), 10 μM BIO, 80 μM SU5402, a combination of 80 μM SU5402 + 10 μM BIO, 10 μg/ml cycloheximide (CHX) or CHX +10 μM BIO, and analyzed for nkx2.1 at stage 35 and sftpc at stage 40. Black arrows indicate normal expression, red arrows indicate absent expression, yellow arrows indicate reduced expression and green arrows indicate expanded or rescued expression. |
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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
sox2
|
|
laevis
|
NF stage 32
to
NF stage 33 and 34
|
otic vesicle
,
brain
,
spinal cord
,
head region
,
endoderm
,
[+]
|
nkx2-1
|
|
laevis
|
NF stage 32
to
NF stage 35 and 36
|
brain
,
forebrain
,
endoderm
,
pharyngeal arch
,
eye
,
[+]
|
sftpc
|
|
laevis
|
NF stage 42
|
lung
,
lung primordium
|
wnt2b
|
|
laevis
|
NF stage 33 and 34
|
otic vesicle
,
pharyngeal arch
,
pineal gland
,
lens placode
,
lateral plate mesoderm
|
|
|
Fig. 5. Osr1/Osr2 act upstream of Wnt-mediated pulmonary specification and RA-regulated lung bud growth. (A-G) Osr1/Osr2-MO- and MM-MO-injected embryos were treated at stages 25-35 with DMSO vehicle, 10 μM BIO or 1 μM all-trans-retinoic acid (RA), and analyzed for nkx2.1, sox2, wnt2b and raldh2 at stage 35 (A-F) and sftpc at stage 42 (dorsal view) (G). Black arrows indicate normal expression, red arrows show absent expression, green arrows and lines indicate expanded expression and white arrows indicate rescued expression. |
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aldh1a2 (aldehyde dehydrogenase 1 family member A2) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 34/35, lateral view, anterior left, dorsal up.
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