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Figure 4. SU5402 treatment effectively knocks down FGF signalling in stage-12 and -16 Xenopus laevis embryos as evidenced by decreased XSpry1 expression. A-D: Stage-12 (A, C) and stage-16 (B, D) Xenopus laevis embryos were incubated overnight (18-22 hr) at 16 in a control dimethyl sulfoxide (CNT; A,B) or a 200- mu M SU5402 (SU5402; C, D) solution and then processed in parallel for XSpry1 expression by in situ hybridization. Note: The stage indicated at the bottom right of each image refers to the age of the embryos at the beginning of their treatment not the stage of the embryos in the photo (embryos that started treatment at stage 12 developed until stage 21, and those that began at stage 16, reached stage 25). E, F: Embryos were scored from 1 to 3 with 1 indicating the lightest or no staining and 3 indicating the darkest level of staining, and the results are graphed as a percentage of embryos that received each score. The number of embryos in each group are as follows: (E) stage-12 CNT, n = 20, stage-12 SU5402 treated, n = 19, (F) stage-16 CNT, n = 20, stage-16 SU5402, n = 19. |
