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Gene/Clone	Species	Stage	Anatomy Item	Experimenter
dnd1	xenopus		foregut endoderm [+] acinar cell,alpha cell,anterior foregut,antrum,beta cell,capillary system of liver,cardia,delta cell,distal stomach,dorsal foregut,dorsal pancreatic bud,epsilon cell,exocrine pancreas,first pancreatic bud,foregut epithelium,fundus,gall bladder,gall bladder primordium,gamma cell,hepato-pancreatic progenitor cell,hepatocyte,islets of Langerhans,liver diverticulum,liver parenchyme,liver primordium,lung bud,lung primordium,posterior foregut,proximal stomach,pulmonary epithelium,pyloric sphincter,pylorus,second pancreatic bud,stomach,stomach body,stomach epithelium,tracheoesophageal fold,tracheoesophageal septum,transient tracheoesophageal septum,ventral foregut,bile duct,biliary tree,buccopharyngeal membrane,dorsal pancreatic bud,first pancreatic bud,foregut,foregut epithelium,gall bladder,gall bladder primordium,hepato-pancreatic duct,hepato-pancreatic progenitor cell,islets of Langerhans,liver,liver diverticulum,liver parenchyme,liver primordium,lung,lung bud,lung primordium,oral evagination,pancreas,pharyngeal cavity,pyloric sphincter,second pancreatic bud,stomach,stomach epithelium,taste bud,thyroid,thyroid primordium,ventral pancreatic bud,visceral pouch

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Experiment details for dnd1

Horvay K et al. (2006) Assay

Xenopus Dead end mRNA is a localized maternal determinant that serves a conserved function in germ cell development.

Gene	Clone	Species	Stages	Anatomy
dnd1.L		laevis	NF stage 15 to NF stage 19	foregut endoderm

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Fig. 1. Localization of XDead end mRNA in Xenopus oocytes and embryos. The subcellular distribution of the XDead end mRNA was analyzed using albino oocytes or embryos of different stages for whole mount in situ hybridization. In stage I and II oocytes, XDead end RNA is homogeneously distributed (A). In later stages (early and late stage III, stage IV) (A) and stage V and VI (B), the XDead end RNA becomes enriched at the vegetal cortex. (D, E) Two-cell stage embryo exhibiting a granular structure at the vegetal tip of both blastomeres. (F) Vegetal view of an 8-cell stage embryo (G) Vegetal view of a gastrula embryo with XDead end positive cells in the center of the yolk plug (marked by an arrow). (H) Transverse section of a neurula stage embryo exhibiting XDead end expression in scattered cells linking the future gut. (I, I′) XDead end expression in migrating germ cells of a tailbud stage embryo. (J) Temporal expression during embryogenesis and tissue expression of XDead end. RT-PCR analysis with RNA preparations from staged embryos (according to Nieuwkoop and Faber, 1967) and adult tissue respectively (sc, spinal cord; mu, muscle; li, liver; he, heart; in, intestine; sk, skin; pa, pancreas; br, brain; ey, eye; lu, lung; te, testis; ov, ovary). Control RT-PCR reactions with primers specifically for Histone 4 (H4).