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Fig. 3. Spatial expression of xlk2 in developing hind limbs. (A) Whole mount in situ hybridization was performed on developing hind limbs of tadpoles at the indicated stages with the antisense probe. The figures represent views of the dorsal side of the limb. (B) A control experiment of A using the sense probe. (C) A section of the limb at the stage 55 shown in A. The arrow indicates the dorsoventral direction. The arrowheads point to the regions where xlk2 was not expressed. (D) Magnification of the region enclosed by the rectangle in C. (E) A serial section of D was stained with hematoxylin. The insets in D and E show magnified figures of the areas enclosed by rectangles. a, the apical layer; b, the basal layer; c, collagen layer; and m; mesenchyme. The magnification of B and E is identical to A and D, respectively. Scale bars = 1 mm in A, and 100 μm in C and D. |
