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Fig. S1. X. tropicalis katnal2cloning and early embryonic expression. A) Full-length katnal2 cDNAs, generated by RT-PCR, migrate as 2 distinct bands above 1.5 kb. B) Nucleotide and protein sequences of the 2 alternatively spliced X. tropicalis katnal2 isoforms, bearing the typical Walker motif A (boxed) and an N-terminal LiSH domain (underlined). The grey shaded area (32 amino acids) corresponds to sequence encoded by exon 6 that is absent from the shorter isoform. X. tropicalis katnal2 sequences were submitted to Genbank with accession numbers MH036373 (larger isoform) and MH036374 (shorter isoform). C) Semi-quantitative RT-PCR of katnal2 throughout X. tropicalis development, compared to drosha and smn2. Note that two isoforms of katnal2 are present in the two-celled embryo and at all later stages sampled (lower expanded panel). D-G) Whole mount RNA in situ hybridization for katnal2 in early X. tropicalis embryos. D) Lateral view of katnal2 expression in the animal hemisphere. E-G) Animal view of maternal katnal2 expression in developing embryos (stages 6–9), compared to sense negative control probe control at stage 9 (H). |
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katnal2 (katanin p60 subunit A-like 2 ) gene expression in Xenopus tropicalis embryo, assayed via in situ hybridization, NF stage 1, horizontal view, animal up. |
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katnal2 (katanin p60 subunit A-like 2 ) gene expression in Xenopus tropicalis embryo, assayed via in situ hybridization, NF stage 9, animal view. |
