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Fig. S2.
Analysis of Gtpbp2 temporal expression by quantitative RT-PCR and whole mount in situ hybridization. (A) Gtpbp2 transcripts were quantified across developmental stages, with expression levels normalized to the housekeeping gene ornithine decarboxylase (odc) and shown in arbitrary units by setting the expression level of a fertilized egg (Stage1) to 1. Gtpbp2 expression significantly decreases during gastrulation. Stage 11 corresponds to mid-gastrulation and stage 12 corresponds to late gastrulation. (B) Whole mount in situ hybridization on staged embryos, confirming the localization of gtpbp2 transcripts to the animal pole of the egg and early cleaving 4-cell blastula. Neurula stage expression is weak but enriched in neural folds at early (stage 15) and late (stage 20) neurula. Stage 25, early tailbud tadpole expression of gtpbp2 is most prominent in the somites (s) and eye (e), with enrichment in the central nervous system (cns). A sense strand RNA in situ probe was hybridized to the tadpole at top, and an antisense probe was hybridized to the tadpole at bottom. |
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Fig. 2.
Temporal and tissue specific expression of Gtpbp2 during Xenopus laevis development. (A) Maternal gtpbp2 transcripts were detected primarily in the animal pole at blastula stages (in sectioned stage 7 embryos). (B) At the onset of gastrulation (stage 10) gtpbp2 is expressed in the inner layer of ectoderm and mesoderm. Arrow shows dorsal blastopore lip. (C) At swimming tadpole stage (stage 35) gtpbp2 is strongly expressed in the somites, as well as in the brain, branchial arches, and ventral blood islands (VBI). Arrow shows VBI-specific expression of gtpbp2. (D) Semi-quantitative RT-PCR showing gtpbp2 levels across stages. The gtpbp2 signal significantly diminishes as gastrulation progresses, but is re-expressed at tadpole stages. |
