|
|
| |
Fig. 2. Immunohistochemistry on seri.
al sections of a stage 7 embryo. In
stage 7 embryos (treated as for Fig. 1J.
mesectoderm is positIVe. whereas endoderm
is not labeled. (al Ectoderm; (b.cl
marginal zone; (dl endoderm. Bar, 50
jJm. |
|
Display additional annotations [+]
|
| |
Fig. 5. Colocalization
of a, subunit of L-type
Ca2+ channel and a
subunit of Go protein.
Animal cap of stage 7
(a), stage 8 (bl and
stage 10 Ic) were dissected
and ceffs dissociated
in a Ca2+/Mg2+
free medium before fixing.
Double labeling on
the same cell was carried
out as described in
Experimental procedures.
Left panel: a,
immunolabeling; right
panel: GaD immunolabeling.
Note that the
two different antibodies
labeled the same region
of the plasma membrane.
However some
slight differences may
exit in the colocalization.
Arrowhead in fb)
poinrs to a I positive and
GaD negative domains.
Conversely arrow in (c)
poinrs to aJ negative
and GaD positive
domains_ Bar. 35 pm. |
|
|
| |
Fig. 3.lmmunolabeling at stage 10'/4 Stage-l01/4 embryos were treated as
described in Fig. 1. Ectoderm and mesoderm are labeled, whereas endoderm
is negative. There is no difference between the dorsal and ventral sides of
the embryo. (a) Ectoderm; (b) ventral marginal zone; (e) endoderm; (d) dorsal
marginal zone. Note the appearance of the blastopore lip (bl). Bar. 50/..lm. |
