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Gene/Clone	Species	Stage	Anatomy Item	Experimenter
clk2	xenopus		otic placode [+] amphibian papilla,anterior crista,auditory hair cell,auditory ossicle,auditory tube,basilar papilla,endolymphatic duct,footplate of the stapes,inner ear,lagena,lateral crista,middle ear,osseous labyrinth,otic capsule,oval window,pars externa of the stapes,pars interna of the stapes,pars media of the stapes,posterior crista,prootic bone,saccular macula,semicircular canal,stapes,tympanic annulus,tympanic disk,vestibular apparatus,vestibule,auditory apparatus,otic capsule,otic vesicle,vestibulocochlear ganglion

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Experiment details for clk2

Cdc2-like kinase 2 (Clk2) promotes early neural development in Xenopus embryos.

Cdc2-like kinase 2 (Clk2) promotes early neural development in Xenopus embryos.

Gene	Clone	Species	Stages	Anatomy
clk2		tropicalis	NF stage 20	otic placode
clk2		tropicalis	NF stage 23	otic placode
clk2		tropicalis	NF stage 29 and 30	otic vesicle

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Figure 2. Expression of clk2 during early development of X. tropicalis. (a) RT‐PCR analysis of temporal expression of clk2. “−RT” indicates RNA of whole embryos (stage 20) examined in the absence of reverse transcriptase. histone h4 was used as the loading control. (b–h) Spatial expression of clk2 analyzed by whole‐mount in situ hybridization. clk2 was expressed in the animal region (b), and only a faint signal was detected in the vegetal region at the blastula stages (c). During gastrula stages (d), the expression of clk2 was localized to the dorsal region (arrowheads). clk2 expression was detected in neural tissues at the neurula stage (e and f). Expression of clk2 was also found in eyes, otic vesicle and branchial arches of tailbud stage embryos (g and h). (b) animal view. (c and d): vegetal view. (e–g) dorsal view with the anterior to the left. (h) lateral side view with the anterior to the left. Embryonic stages were determined according to Nieuwkoop and Faber (1967).

Gene	Clone	Species	Stages	Anatomy
clk2		tropicalis	NF stage 20	otic placode

clk2 (cdc2‐like kinase 2) gene expression in Xenopus tropicalis embryo, assayed via in situ hybridization, NF stage 20, dorsal view, anterior left.

Gene	Clone	Species	Stages	Anatomy
clk2		tropicalis	NF stage 23	otic placode

clk2 (cdc2‐like kinase 2) gene expression in Xenopus tropicalis embryo, assayed via in situ hybridization, NF stage 23, dorsal view, anterior left.

Gene	Clone	Species	Stages	Anatomy
clk2		tropicalis	NF stage 29 and 30	otic vesicle

clk2 (cdc2‐like kinase 2) gene expression in Xenopus tropicalis embryo, assayed via in situ hybridization, NF stage 29 & 30, lateral view, anterior left, dorsal up.