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Fig. 1. Pattern of hsp30 mRNA accumulation during early Xenopus development. Whole mount in situ hybridization with DIG-labeled hsp30 antisense
riboprobe was carried out with control (A) and heat shocked (B) (1 h at 33 ◦C) Xenopus midtailbud stage embryos. Preferential heat-induced accumulation
of hsp30 mRNA was observed in lens placode (LP), somites (S), proctodeum (P) and cement gland (CG). Constitutive hsp30 mRNA accumulation was
also observed in cement gland. (C) Treatment of Xenopus embryos with HDIs resulted in premature heat-induced accumulation of hsp30 mRNA at the
gastrula stage rather than at the late neurula–early tailbud stages as found in non-HDI-treated embryos. HDI treatment enhanced heat-induced hsp30
mRNA accumulation in neurula and tailbud embryos. These experiments suggest that hsp30 gene expression during development is regulated at the level
of chromatin structure. Xenopus embryos were incubated with (+) or without (−) 30 nM trichostatin A (TSA), 5mM valproic acid (VPA), or 5mM
sodium butyrate (NaB). Total RNA was isolated from control (C; 22 ◦C) and heat shocked (H; 1 h at 33 ◦C) embryos and subjected to Northern blot
analysis using an hsp30 antisense riboprobe. Arrows indicate the positions of hsp30 mRNA. |
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hsp30e (heat shock protein 30E) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 28, lateral view, anterior left, dorsal up. |
