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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
spry1
|
|
laevis
|
NF stage 50
to
NF stage 53
|
anterior
,
hindlimb bud
,
distal mesenchyme
|
spry4
|
|
laevis
|
NF stage 50
to
NF stage 51
|
hindlimb bud
,
distal mesenchyme
|
spry4
|
|
laevis
|
NF stage 52
|
blood vessel
,
hindlimb bud
,
distal mesenchyme
|
spry4
|
|
laevis
|
NF stage 53
|
blood vessel
,
hindlimb bud
,
distal mesenchyme
,
autopod
,
hindlimb stylopod
,
[+]
|
spry4
|
|
laevis
|
NF stage 54
|
hindlimb bud
,
distal mesenchyme
,
hindlimb digit 3
,
hindlimb digit 4
|
spry4
|
|
laevis
|
NF stage 55
|
blood vessel
,
hindlimb bud
,
distal mesenchyme
,
autopod
,
hindlimb digit 3
,
[+]
|
fgf8
|
|
laevis
|
NF stage 50
to
NF stage 54
|
hindlimb bud
,
apical ectodermal ridge
,
hindlimb bud tip
|
spry2
|
|
laevis
|
NF stage 50
to
NF stage 51
|
mesenchyme
,
hindlimb bud
,
distal mesenchyme
|
spry2
|
|
laevis
|
NF stage 52
|
blood vessel
,
hindlimb bud
,
distal mesenchyme
,
apical ectodermal ridge
,
autopod
,
[+]
|
spry2
|
|
laevis
|
NF stage 53
to
NF stage 54
|
blood vessel
,
hindlimb bud
,
distal mesenchyme
,
apical ectodermal ridge
,
autopod
,
[+]
|
|
|
Fig. 2. In situ hybridisation of spry1a, spry2, spry4 and fgf8b in Xenopus laevis developing hindlimbs from stage 49 to 55. Fgf8b is included to show the location of the AER at developmental stages 50–54. Specific staining is dark purple. Black spots in some panels are melanophores in pigmented specimens. Black arrows indicate distal expression, red arrows localised expression in more proximal mesenchyme of older limbs, and thin white arrows indicate possible forming blood vessels. Roman numerals show detectable staining in some digits. The AER is indicated in some panels to show absence of spry1a and spry4 and presence of spry2 and fgf8b transcripts. All limbs are oriented proximal to the left and posterior uppermost, with the tadpole on its right side, except for panels B, I and V where both hind limbs are viewed from the anterior with the tadpole placed on its back. For stage 54 and 55 limbs only the autopod is shown for spry1a, spry4 and fgf8b, as by this stage there is no proximal staining. Developmental stage is in the bottom right of each panel and limbs are photographed to the same scale, except for panel T which shows histological section through a stage 53 limb bud to show expression of spry2 in the AER around digit IV. Scale bar in T is 50 um. |
|
|
|
spry1 (sprouty homolog 1, antagonist of FGF signaling ) gene expression in Xenopus laevis embryonic hindlimb, assayed via in situ hybridization, NF stage 55, proximal to the left and posterior uppermost, with the tadpole on right side. |