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strap (serine/threonine kinase receptor associated protein) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 18, dorsal view, anterior left. |
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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
hoxd4
|
|
laevis
|
NF stage 19
to
NF stage 20
|
spinal cord
|
pax6
|
|
laevis
|
NF stage 19
to
NF stage 20
|
optic vesicle
|
otx2
|
|
laevis
|
NF stage 19
to
NF stage 20
|
forebrain
|
en2
|
|
laevis
|
NF stage 19
to
NF stage 20
|
presumptive midbrain-hindbrain boundary
|
egr2
|
|
laevis
|
NF stage 19
to
NF stage 20
|
hyoid crest
,
rhombomere R3
,
rhombomere R5
|
hoxb9
|
|
laevis
|
NF stage 19
to
NF stage 20
|
spinal cord
|
strap
|
|
laevis
|
NF stage 10.5
to
NF stage 11
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marginal zone
|
strap
|
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laevis
|
NF stage 14
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pre-chordal neural plate
,
chordal neural plate
,
neural plate
|
strap
|
|
laevis
|
NF stage 18
|
mandibular crest
,
hyoid crest
,
branchial crest
,
optic vesicle
,
posterior neural tube
,
[+]
|
strap
|
|
laevis
|
NF stage 29 and 30
|
brain
,
pharyngeal arch
,
mandibular arch
,
hyoid arch
,
branchial arch
,
[+]
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Figure 6. STRAP knockdown influences anterior‐posterior neural patterning and impairs the body axis and eye de‐
velopment in early Xenopus embryogenesis. A. Strap transcript was detected in the entire blastopore (a), early neu‐
rulation (b), late neurula stage (c), and the tailbud and the tadpole stages (d and e, respectively). B. 50 ng Strap‐MO
was injected into the animal regions of two‐cell‐stage embryos and resulted in defects in the tadpole stage. C. Strap
RNA (0.20 ng) was injected alone or with 25 ng Strap‐MO unilaterally into two‐cell‐ stage embryos. The embryos were
stained with the β‐Gal substrate Red‐Gal. D. 50 ng Strap‐MO was injected unilaterally into two‐cell‐ stage embryos.
The embryos were examined at neurula stages 17‐18 by ISH for neural markers. E. 50 ng Strap‐MO was injected into
the animal regions of two‐cell‐stage embryos. Animal caps were dissected at blastula stage 9 and cultured until neu‐
rula stages 19‐20. RNA was then extracted for RT‐qPCR analysis of marker expression. Bars represent mean ±s.d., n=3.
* P<0.05, **P<0.01, when compared with the control. |