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Figure 4
N-cadherin Binding Leads to Src Activity at the Contact
(A) Western blot against total FAK and pY861 FAK in control or N-cadherin MO cells.
(B) Quantification of (A); n = 3 repeats.
(C and D) Cell clusters for Src-FRET as indicated.
(E and F) Src-FRET efficiency as indicated. n = 23 cells for each condition.
(G–I) Immunocytochemistry against active Src (green) in cells injected with membrane RFP (red) and stained with Hoescht (blue) as indicated. Box shows region of zoom over contact.
(J–L) Intensity of active Src at contact and free edge as indicated. Control, n = 125; N-cadh MO, n = 60; N-cadh BA, n = 64.
Scale bar, 20 μm. Line graph shows means, errors ± SEM; bar graphs show means; error bars are ±SEM. ∗∗∗p ≤ 0.001, ∗∗p ≤ 0.01, ∗p ≤ 0.05. (B, F, and L) t test, (E and J) paired t test. |
