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Fig. 1. NC cells express both PDGF-A and
PDGFRα. (A-I) Whole-mount in situ
hybridization of Xenopus embryos.
(A,D,G) Lateral view of stage 18 embryos
showing expression of (A) slug, (D) pdgfrα and
(G) pdgf-a. (B,E,H) Lateral view of stage 24
embryos showing migrating NC expressing
(B) twist, (E), pdgfrα and (H) pdgf-a. Scale bar:
1 mm. Grey stars indicate the eyes.
(C,F,I) Sections of embryos shown in B,E,H,
respectively. The line in B indicates the level of
the section. Yellow dashed lines outline
cephalic NC streams. (J) RT-PCR analysis of
pdgf-a and pdgfrα expression in NC dissected
from stage 18 embryos (premig. NC) and whole
embryos along with Sox9 (NC marker), Sox2
(neural plate marker), brachyury (mesoderm
marker), e-Keratin (epidermis marker) and
ODC (control, ornithine decarboxylase).
(K) Immunostaining for PDGFRα (green),
Phalloidin (red) and DAPI (blue) in NC explants.
Scale bars: 20 μm. (L) Western blot analysis of
PDGFRα in NC dissected from control embryos,
embryos treated with PDGF-A or embryos
injected with PDGFRα MO. GADPH was used
as a loading control. (M) Band intensity
normalized to the loading control. Data are
mean±s.d. of three independent experiments.
AU, arbitrary units. ns, not significant;
***P<0.001. |
