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Fig. 5. Localization of integrin aS mRNA during embryogenesis. Whole mount in situ hybridization with digoxygenin-rUTP-labeled antisense
riboprobes was used to determine the localization of integrin q mRNAs throughout development. In each panel, arrows indicate expression in
neural crest cells and arrowheads point to staining within the somitic mesoderm. (A) Dorsal view, stage 1 I .5-12 gastrula, oriented with anterior
end at the top of the panel. (B) Optical section of (A). The approximate plane of section is indicated by the line in (A). (C) Frontal view, stage
15 neurula, dorsal to the top. Integrin q transcripts are detected in the neural crest (arrow) and paraxial mesoderm (arrowhead). (D) Side view,
stage 14 neuruia, anterior to the right. (E) Optical section, stage 17 neurula, dorsal is to the top. Arrow indicates trunk neural crest cells, arrowhead
marks the somitic mesoderm. (F) Dorsal view, stage 19 neurula, anterior to the left. Cranial neural crest cells (black arrow) are intensely stained
and located lateral to the unstained neural tube. Trunk neural crest forms a single line of cells along the dorsal midline of the embryo (white arrow).
(G) Side view, stage 21 neurula, anterior to the right and dorsal at the top. The somatic staining of the integrin os is concentrated around the nuclei
of the post-rotation somites (arrowhead). Intensely stained cranial neural crest cells are visible in the anterior part of the embryo (arrow). (H) Optical
section, stage 22 neurula, dorsal to the top. Trunk neural crest a single line of cells dorsal to the neural tube (arrow). Staining within the somites
is also visible (arrowhead). (I) Dorsal view, stage 23 neurula, anterior to the top. Staining is localized in discrete foci in the post-rotation somitic
mesoderm (black arrowhead) and is diffuse in the pre-rotation mesoderm (white arrow). (J) Side view, stage 32 tailbud embryo, anterior to the
right. Note weaker staining in anterior somites. (K) High magnification view of the tailbud of the embryo in (J). Integrin (r5 expression in the tail
remains restricted to the neural crest (arrow) and somites (arrowhead). |
