| |
Supplementary Figure 4: hmg20a expression in X. laevis.
(A) Alignment of human (H. sapiens), mouse (M. musculus) and frog (X. laevis) Hmg20a protein sequences. HMG box is shown in blue, coiled-coil region in red. Alignment was performed using Clustal Omega.
(B) Temporal expression pattern of Xenopus hmg20a: RT-qPCR of hmg20a mRNA expression covering X. laevis developmental stages 4 (8-cell stage) to 42 normalized to odc expression. Error bars indicate s.e.m. of three technical replicates.
(C-R’) Spatial expression pattern of hmg20A determined by whole mount in situ hybridization. hmg20A mRNA is detected at early stages of Xenopus laevis development. (C) 8-cell stage embryo, anterior view. (D) 8-cell stage embryo, dorso- lateral view, animal and vegetal pole are indicated. (E) Embryo at blastula stage 6.5. anterior view. (F) Same embryo as in E, dorsal view. (G) Embryo at gastrula stage 10. (H) Embryo at neurula stage 19, anterior view. (I) Same embryo as in H, dorsal view. (J) Embryo at stage 20, lateral view. (K) Embryo at stage 24, lateral view. (L) Sense control, embryo at stage 24. (M) Embryo at stage 33, lateral view. (N) Sense control, embryo at stage 33. Scale bar in C-N is 1mm. (O) Transverse section through the branchial arch region of a stage 31 embryo, hmg20A expression in the branchial arches is indicated by arrows. (P-R’) Transverse sections of a stage 42 embryo. (P) hmg20A is partially expressed in the heart region. (Q) hmg20A expression within the notochord (no). (R, R’) hmg20A is partially expressed in the brain and eye. Scale bar in O-R’ is 100 μm. abbreviations: a, atrium, an, animal; b, brain; ba, branchial arches; bl, blastoporus; ea, eye anlage; e, eye; inl, inner nuclear layer; le, lens; mn, migratory neural crest; nf, neural fold; no, notochord; ov, otic vesicle; rpe, retinal pigment epithelium v, ventricle; ve, vegetal.
Source data for these figures are provided as a Source Data file. |
