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Fig. 4. Expression pattern of zcchc24 during tailbud stages. Whole mount in situ hybridization using DIG labelled zcchc24 (A, B, F-G’), fluorescein labelled nkx2.5 (C,H) or fluorescein labelled twi (D,J), and double whole mount in situ hybridization using DIG labelled zcchc24 and fluorescein labelled twi (E,K) or DIG labelled zcchc24 and fluorescein labelled nkx2.5 (I) were performed on embryos at tailbud stages. (A-E) At early tailbud stages, zcchc24 is strongly expressed in the unsegmented somitogenic mesoderm, somites, and migrating neural crest but is absent in the region of differentiating cardiac muscle. (F) Later, at st 28, zcchc24 transcripts start to be detected in the forming heart, and in the entire head except the region between the branchial arches. (G-K) This expression is maintained throughout tailbud development, being zcchc24 expressed in the somites, branchial arches, head and heart. All the embryos are displayed in a lateral view with the anterior region to the left. (G’) Transversal section of the heart region of a st 32 embryo. The orange arrows indicate the neural crest/branchial arches, and the red arrows indicate heart-forming region/heart. A, anterior; P, posterior; ec, endocardium; pc, pericardium; mc, myocardium; bam, branchial arch mesenchyme; cg, cement gland. |
