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Supplemental Figure II. Expression of Shox2 in Xenopus laevis. (A) Developmental RT-PCR of
Xenopus embryos with indicated Nieuwkoop and Farber (N.F.) stages. Ornithine decarboxylase
(ODC) was used as a control to ensure an equal amount of RNA in each RT-PCR reaction. As a
negative control, PCR on the same RNA prior to reverse transcription was performed to exclude a
genomic DNA contamination (-RT). RT-PCR analysis shows that Shox2 specific transcripts are first
detected at stage 23 and expression persists up to stage 45. (B-D) Spatial expression of Shox2 during
Xenopus laevis development detected by whole mount in situ hybridisation on endogenous Shox2
RNA at different stages (B-D). Shox2 transcripts are localised in the mesencephalon (black
arrowheads) and in the heart (red arrows). (E-H) Transverse sections (30μm) through the heartforming
region of a stage 31 embryo after whole mount in situ hybridisation. Positions of sections are
indicated by white dotted lines (C) and letters refer to the corresponding panels. The most anterior
section (E) shows endocardial, myocardial and pericardial cell layers. In more posterior sections (F-H)
Shox2 expression is detected in myocardial tissue. e: endocard, m: myocard, p: pericard, liv: liver
primordium. |
