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Figure 5. Histologic analysis of embryos stained for somitic markers. All sections are transverse, through the middle of the trunk. A-D: Embryos stained for koza at late neurula (stage [st.] 28, A), early tail bud (st. 32, B), late tail bud (st. 40, C), and mid-tail bud (st. 37, D). Note the progressively more restricted pattern of koza expression. D: koza transcripts are visible in the myocardial layer of the heart (arrow) but not in the endocardium (arrowhead). E: Early tail bud embryo (st. 32) stained for MHC E19. Note that the pattern of expression is very similar to that of a similarly staged embryo stained for koza (B). F: Late tail bud embryo (st. 40) probed for myosin light chain (MLC). In contrast to koza at this stage (C), MLC is expressed throughout the myotome. Scale bars = 200 microns in A-F. |
