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Figure 2. Developmental expression of myh7b in Xenopus and chick. In situ hybridization analysis was used to detect myh7b transcripts. (A) myh7b expression was first detected in the somites of the neurula stage frog embryo, (B) At the early tailbud stage, transcripts were observed in developing heart tissue (h) and in the perinuclear region of somite cells (som�arrowheads). (C) Ventral view of the frog embryo showed expression in the paired heart patches (arrows) prior to fusion at the ventral midline. (D). During later development, myh7b expression persisted in the heart (h) but was reduced in the somites (E) Chick embryo at HH12, showing MYH7B expression in the folding heart tube (h). (F). During later development, transcripts persisted in the heart (h) and became visible in the somites (arrowheads). (G) Longitudinal section through the heart of a D19.5 chick embryo. MYH7B transcripts were detected throughout the myocardial layer. (H). Magnified view of atrioventricular septal region of D19.5 heart, showing expression of MYH7B in myocardium (myo) but absence from developing valve tissue (val). (I). Magnified view of the surface of the D19.5 heart, showing absence of MYH7B expression in the epicardial tissue layer (epi).
Copyright 2012, John Wiley & Sons. |
