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Gene/Clone	Species	Stage	Anatomy Item	Experimenter
vangl2	xenopus		involuted dorsal mesoderm [+] Eberth-Kastschenko layer,abdominal myoblast,anterior notochord,archenteron roof midline,axial ossicle,caudal presomitic mesoderm,cranial skeleton,dermatome,dermomyotome,dorsal endoderm,dorsomedial edge of myotome,flexural organ,left-right organizer,left-right organizer primordium,mandibular mesenchyme,monociliated cell,myocoelic slit,myotome,notochordal sheath,perichordal tissue,postcranial axial skeleton,posterior notochord,prechordal plate,presomitic mesoderm,rib,rostral presomitic mesoderm,sclerotomal cartilage,sclerotomal mesenchyme,sclerotome,stratum compactum,stratum spongiosum,syndetome,transverse process,urostyle,vacuolated notochordal tissue,ventrolateral edge of myotome,vertebra,vertebral centrum,vertebral column,vertebral element,axial mesoderm,axial skeleton,cervical vertebra,dermatome,dermis,elastica externa,flexural organ,gastrocoel roof plate,head somite 1,head somite 2,head somite 3,head somite 4,myotome of head somite 1,myotome of head somite 2,myotome of head somite 3,myotome of head somite 4,myotome of tail somite 14,myotome of tail somite 15,myotome of tail somite 16,myotome of tail somite 17,myotome of tail somite 18,myotome of trunk somite 1,myotome of trunk somite 10,myotome of trunk somite 11,myotome of trunk somite 12,myotome of trunk somite 13,myotome of trunk somite 2,myotome of trunk somite 3,myotome of trunk somite 4,myotome of trunk somite 5,myotome of trunk somite 6,myotome of trunk somite 7,myotome of trunk somite 8,myotome of trunk somite 9,notochord,notochordal epithelium,paraxial mesoderm,presomitic mesoderm,presumptive axial mesoderm,presumptive paraxial mesoderm,sacral vertebra,sclerotome of head somite 4,sclerotome of tail somite 14,sclerotome of tail somite 15,sclerotome of tail somite 16,sclerotome of tail somite 17,sclerotome of tail somite 18,sclerotome of trunk somite 1,sclerotome of trunk somite 10,sclerotome of trunk somite 11,sclerotome of trunk somite 12,sclerotome of trunk somite 13,sclerotome of trunk somite 2,sclerotome of trunk somite 3,sclerotome of trunk somite 4,sclerotome of trunk somite 5,sclerotome of trunk somite 6,sclerotome of trunk somite 7,sclerotome of trunk somite 8,sclerotome of trunk somite 9,somite,tail somite 14,tail somite 15,tail somite 16,tail somite 17,tail somite 18,trunk somite 1,trunk somite 10,trunk somite 11,trunk somite 12,trunk somite 13,trunk somite 2,trunk somite 3,trunk somite 4,trunk somite 5,trunk somite 6,trunk somite 7,trunk somite 8,trunk somite 9,urostyle

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Experiment details for vangl2

The planar cell polarity gene strabismus regulates convergence and extension and neural fold closure in Xenopus.

The planar cell polarity gene strabismus regulates convergence and extension and neural fold closure in Xenopus.

Gene	Clone	Species	Stages	Anatomy
vangl2.L		laevis	NF stage 10 to NF stage 10.5	involuted dorsal mesoderm
vangl2.L		laevis	NF stage 28	notochord

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FIG. 2. Temporal and spatial expression of Xstbm. (A) Quantitative RT-PCR was performed by using 1 g of total RNA extracted from Xenopus embryos at different stages. “U” indicates the unfertilized eggs, and numbers indicate the developmental stages according to Nieuwkoop and Faber (1967). Maternal transcripts decreased gradually until the gastrula stage (stage 10), and zygotic expression increased thereafter in the gastrula (stage 12) and neurula (stage 15–20). (B–O) Localization of Xstbm transcripts by whole-mount in situ hybridization. At the gastrula stage, Xstbm is expressed in the dorsal region (B, dorsal view, stage 10; C, lateral view, stage 12). Xstbm is expressed in the neural plate of the early neurula (D, stage 14) and late neurula (E, stage 17), and in the neural tube thereafter in the tailbud stages (F, stage 20; G, stage 25, dorsal view; H, stage 25, lateral view; I, stage 30, lateral view). At tailbud stage, Xstbm is also expressed in prenephritic region (I, yellow pointer). Sections of the gastrula (B, C) show low expression at the dorsal lip (red pointer) and higher expression in the posterior mesodermal–neural region (yellow pointer) above the blastoporal lip (J, stage 10). The late gastrula shows high expression in the prospective forebrain (K, stage 12, yellow pointer) and low expression in the anterior mesodermal region (K, red pointer). The superficial epithelial layer of the involuting mesodermal region showed little or no expression at stage 10 (J, white pointer) and stage 12 (K, white pointer). The posterior mesodermal regions of a dorsal sandwich explant showed expression at the late neurula stage (L, yellow pointer), but expression declined anteriorly (L, red pointer). Transverse sections of a tailbud stage embryo in (I) showed the expression of Xstbm increased progressively from the anterior (M, yellow pointer) to the middle (N, yellow pointer) and posterior notochord (O, yellow pointer).