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Gene/Clone	Species	Stage	Anatomy Item	Experimenter
pld1	xenopus		involuted dorsal mesoderm [+] Eberth-Kastschenko layer,abdominal myoblast,anterior notochord,archenteron roof midline,axial ossicle,caudal presomitic mesoderm,cranial skeleton,dermatome,dermomyotome,dorsal endoderm,dorsomedial edge of myotome,flexural organ,left-right organizer,left-right organizer primordium,mandibular mesenchyme,monociliated cell,myocoelic slit,myotome,notochordal sheath,perichordal tissue,postcranial axial skeleton,posterior notochord,prechordal plate,presomitic mesoderm,rib,rostral presomitic mesoderm,sclerotomal cartilage,sclerotomal mesenchyme,sclerotome,stratum compactum,stratum spongiosum,syndetome,transverse process,urostyle,vacuolated notochordal tissue,ventrolateral edge of myotome,vertebra,vertebral centrum,vertebral column,vertebral element,axial mesoderm,axial skeleton,cervical vertebra,dermatome,dermis,elastica externa,flexural organ,gastrocoel roof plate,head somite 1,head somite 2,head somite 3,head somite 4,myotome of head somite 1,myotome of head somite 2,myotome of head somite 3,myotome of head somite 4,myotome of tail somite 14,myotome of tail somite 15,myotome of tail somite 16,myotome of tail somite 17,myotome of tail somite 18,myotome of trunk somite 1,myotome of trunk somite 10,myotome of trunk somite 11,myotome of trunk somite 12,myotome of trunk somite 13,myotome of trunk somite 2,myotome of trunk somite 3,myotome of trunk somite 4,myotome of trunk somite 5,myotome of trunk somite 6,myotome of trunk somite 7,myotome of trunk somite 8,myotome of trunk somite 9,notochord,notochordal epithelium,paraxial mesoderm,presomitic mesoderm,presumptive axial mesoderm,presumptive paraxial mesoderm,sacral vertebra,sclerotome of head somite 4,sclerotome of tail somite 14,sclerotome of tail somite 15,sclerotome of tail somite 16,sclerotome of tail somite 17,sclerotome of tail somite 18,sclerotome of trunk somite 1,sclerotome of trunk somite 10,sclerotome of trunk somite 11,sclerotome of trunk somite 12,sclerotome of trunk somite 13,sclerotome of trunk somite 2,sclerotome of trunk somite 3,sclerotome of trunk somite 4,sclerotome of trunk somite 5,sclerotome of trunk somite 6,sclerotome of trunk somite 7,sclerotome of trunk somite 8,sclerotome of trunk somite 9,somite,tail somite 14,tail somite 15,tail somite 16,tail somite 17,tail somite 18,trunk somite 1,trunk somite 10,trunk somite 11,trunk somite 12,trunk somite 13,trunk somite 2,trunk somite 3,trunk somite 4,trunk somite 5,trunk somite 6,trunk somite 7,trunk somite 8,trunk somite 9,urostyle

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Experiment details for pld1

PLD1 regulates Xenopus convergent extension movements by mediating Frizzled7 endocytosis for Wnt/PCP signal activation.

PLD1 regulates Xenopus convergent extension movements by mediating Frizzled7 endocytosis for Wnt/PCP signal activation.

Gene	Clone	Species	Stages	Anatomy
pld1.L		laevis	NF stage 10.5	involuted dorsal mesoderm

Fig. 1. PLD1 is dynamically expressed during Xenopus development. (A) Schematic diagrams of PLD1 in various species. PLD1 is well conserved among vertebrates. The number of amino acids in each PLD1 protein is indicated. (B) In situ hybridization assay with PLD1 and sagittal section views. Endogenous PLD1 was expressed in the involuting mesoderm and the overlying ectoderm. A white arrow indicates dorsal lips of stage 10.5 embryos and right panel is a magnified view of a white rectangle. (C) PLD1 protein was asymmetrically enriched in the dorsal region. Dorsal Marginal Zone (DMZ), Ventral Marginal Zone (VMZ), and Animal Cap (AC) were subjected to western blotting with anti-PLD1 antibody.

Gene	Clone	Species	Stages	Anatomy
pld1.L		laevis	NF stage 10.5	involuted dorsal mesoderm

pld1 (phospholipase D1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 10.5, lateral view of bisected embryo,dorsal right.