| |
Figure 1 Localization of 14-3-3ς mRNA in retinal axons and growth cones. (A) RT-PCR of mRNAs purified from heads and eyes of various stages. RT only control was performed to rule out genomic contaminations. (B) Representative images of FISH with either anti-sense or sense probes (scale bar = 5 μm) (R1). (C) Quantification of FISH mean intensity per unit area (n = no. of growth cones; **** = p<0.0001; Mann–Whitney). (D) In situ hybridization of stage 40 embryo sections (ONH = optic nerve head, LS = lens, RGC = retinal ganglion cells, IPL = inner plexiform layer, OPL = outer plexiform layer, OT = optic tectum; scale bars = 25 μm for left panel and 100 μm for the middle panel). |
