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Fig. 3. Spatio-temporal expression pattern of sox14. (A) RT-PCR analysis from oocytes and embryos to stage 38 as indicated across the top. ODC (lower panel) was used as the loading control. (B-I) In situ hybridization of sox14 at indicated stages. In all figures, anterior is to the left. (B) Lateral view; (C) dorsal view, anterior view in the inset. (D) Sagittal sections of stage 38, double in situ of sox14 and en2 in the inset. (E) Dissected brain from stage 38 embryo. (F-I) Transverse sections from stage 32 (I) and 38 (F-H) embryos. Symbols: bracket, expression in vestibular nuclei; black arrowhead, dorsal thalamus; open arrowhead, dorsal hindbrain sox14 positive cells; M, midbrain; hy, hypothalamus; and AM, abducens motornucleus; mhb, midbrain hindbrain barrier; OT, optic tectum; DT, dorsal thalamus; OM, oculomotor motornucleus; TM, trochlear motornucleus; IN, interneurons. Black bar equivalent to 100 μm. |
