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Fig.1 Indirect immunofluorescence of MAb-45 and MAb-B2 on cryostat sections of the optic tectum of Xenopus tadpoles (stage 53). (a) A coronal section of the mesencephalon stained with MAb-A5. Only the outer layers of the optic tectum (OT) show the fluorescence (arrow),
whereas the tegmentum (TG) is negative (X100). (b) A section similar to (a) stained with MAb-BX. Both the optic tectum and tegmentum are
stained. (c) Immunofluorescence with MAb-A5 in the optic tectum is restricted to the 8th and 9th plexiform layers (X160). (d) The section next
to (c) is stained with MAb-B2. The 3rd, 5th, and 7th plexiform layers of the optic tectum show immunofluorescence with MAb-B2, but the 8th
and 9th layers are practically negative. (e) The same section as (d) viewed with bright-field optics. The numbers indicate the 3rd, 5th, 7th, 8th,
and 9th plexiform layers of the optic tectum, respectively. |
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FIG. 7. Immunofluorescent staining of viable tadpole brains with MAb-A5 (a) and MAb-B2 (b) (stage 53). Numbers indicate the 3rd, 5th, 7th,
8th. and 9th plexiform layers of the optic tectum (X140) |
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plxna1 (plexin A1) gene expression in a Xenopus laevis embryo, NF stage 53, as assayed by immunohistochemistry. Coronal sections of brain, dorsal up.
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