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Fig. 7.
Selective effect of NOS inhibition on neuronal differentiation. a–d, Horizontal brain sections of animals treated with saline (Control) andL-NAME for 7 d were immunostained with antibodies to pan-neuronal markers N-tubulin (a, b) and N-CAM (c, d). Excess cells generated as a result of NOS inhibition differentiate and express neuronal markers. Note the gross disorganization of the optic tectum in animals treated with the NOS inhibitor L-NAME. e, f, Coronal brain sections through the brains of animals treated with saline (Control) or L-NAME in Elvax were immunostained with antibodies to Islet-1. Islet 1-positive neurons differentiated before L-NAME treatment was started. Although the overall sizes of the brains of L-NAME-treated animals were larger, and they contained more cells, the pattern and number of the early differentiating Islet-1-positive neurons were not affected by L-NAME treatment, as shown in the representative sections. Rostral (R) is at thetop in a–d; C, caudal; dorsal (D) is at the top ine, f; V, ventral. Scale bar, 50 μm. |
