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Gene/Clone	Species	Stage	Anatomy Item	Experimenter
trpm7	xenopus		involuted dorsal mesoderm [+] Eberth-Kastschenko layer,abdominal myoblast,anterior notochord,archenteron roof midline,axial ossicle,caudal presomitic mesoderm,cranial skeleton,dermatome,dermomyotome,dorsal endoderm,dorsomedial edge of myotome,flexural organ,left-right organizer,left-right organizer primordium,mandibular mesenchyme,monociliated cell,myocoelic slit,myotome,notochordal sheath,perichordal tissue,postcranial axial skeleton,posterior notochord,prechordal plate,presomitic mesoderm,rib,rostral presomitic mesoderm,sclerotomal cartilage,sclerotomal mesenchyme,sclerotome,stratum compactum,stratum spongiosum,syndetome,transverse process,urostyle,vacuolated notochordal tissue,ventrolateral edge of myotome,vertebra,vertebral centrum,vertebral column,vertebral element,axial mesoderm,axial skeleton,cervical vertebra,dermatome,dermis,elastica externa,flexural organ,gastrocoel roof plate,head somite 1,head somite 2,head somite 3,head somite 4,myotome of head somite 1,myotome of head somite 2,myotome of head somite 3,myotome of head somite 4,myotome of tail somite 14,myotome of tail somite 15,myotome of tail somite 16,myotome of tail somite 17,myotome of tail somite 18,myotome of trunk somite 1,myotome of trunk somite 10,myotome of trunk somite 11,myotome of trunk somite 12,myotome of trunk somite 13,myotome of trunk somite 2,myotome of trunk somite 3,myotome of trunk somite 4,myotome of trunk somite 5,myotome of trunk somite 6,myotome of trunk somite 7,myotome of trunk somite 8,myotome of trunk somite 9,notochord,notochordal epithelium,paraxial mesoderm,presomitic mesoderm,presumptive axial mesoderm,presumptive paraxial mesoderm,sacral vertebra,sclerotome of head somite 4,sclerotome of tail somite 14,sclerotome of tail somite 15,sclerotome of tail somite 16,sclerotome of tail somite 17,sclerotome of tail somite 18,sclerotome of trunk somite 1,sclerotome of trunk somite 10,sclerotome of trunk somite 11,sclerotome of trunk somite 12,sclerotome of trunk somite 13,sclerotome of trunk somite 2,sclerotome of trunk somite 3,sclerotome of trunk somite 4,sclerotome of trunk somite 5,sclerotome of trunk somite 6,sclerotome of trunk somite 7,sclerotome of trunk somite 8,sclerotome of trunk somite 9,somite,tail somite 14,tail somite 15,tail somite 16,tail somite 17,tail somite 18,trunk somite 1,trunk somite 10,trunk somite 11,trunk somite 12,trunk somite 13,trunk somite 2,trunk somite 3,trunk somite 4,trunk somite 5,trunk somite 6,trunk somite 7,trunk somite 8,trunk somite 9,urostyle

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Experiment details for trpm7

TRPM7 regulates gastrulation during vertebrate embryogenesis.

TRPM7 regulates gastrulation during vertebrate embryogenesis.

Gene	Clone	Species	Stages	Anatomy
trpm7.L		laevis	NF stage 12	involuted dorsal mesoderm
trpm7.L		laevis	NF stage 15	paraxial mesoderm
trpm7.L		laevis	NF stage 19	paraxial mesoderm , axial mesoderm
trpm7.L		laevis	NF stage 25	paraxial mesoderm , axial mesoderm
trpm7.L		laevis	NF stage 28	paraxial mesoderm
trpm7.L		laevis	NF stage 35 and 36	notochord

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Fig. 1. XTRPM7 is required for gastrulation. (A) Expression pattern of XTRPM7 at selected developmental stages as analyzed by whole-mount in situ hybridization using a TRPM7 anti-sense probe. A sense probe was used as a negative control. XTRPM7 is expressed at higher levels in the dorsal mesoderm and neural plate (stars and arrowheads, respectively), which is associated with dynamic morphogenesis movements. Arrows indicate the region where the embryo was vertically sectioned and examined. AP: Animal pole; VP: Vegetal pole; DV: dorsal view; TS: transverse section; LV: lateral view; ST: stage (B) Injection of TRPM7 RNA (2 ng) dorsally, but not ventrally, inhibited gastrulation resulting in embryos with curved axes, open neural folds, and reduced anterior structures. Dorsal injection of the kinase-dead TRPM71618D (TRPM7D, 2 ng), but not the channel-dead TRPM71047K (TRPM7-CD, 2 ng), produced a similar phenotype. In addition, dorsal injection of TRPM6 (2 ng) and the Mg2+ transporter SLC41A2 (2 ng) RNAs also produced gastrulation defects. Dorsal injection of XTRPM7 MOs (37.5 ng each) inhibited gastrulation, and this phenotype was rescued by co-injection of XTRPM7 MOs with TRPM7 RNA (400 pg), TRPM7-KD RNA (400 pg), and TRPM6 RNA (400 pg) as well as by SLC41A2 (Mg2+ transporter) RNA (400 pg). However, co-injection of TRPM7-CD RNA with the XTRPM7 MOs did not rescue the XTRPM7 MO-induced gastrulation phenotypes. Injections were performed into the dorsal or ventral marginal zone of the four-cell embryo, and the phenotypes were scored at the tadpole stage. (C) Quantification of the phenotypic results from overexpression of TRPM7 or depletion of XTRPM7; the number of embryos examined is shown above each bar.