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Fig. 2. Katnal2 localizes to epidermal cilia, basal bodies, and centrioles. A-B) Katnal2 rabbit antibody staining (green) of X. tropicalis multiciliated cells of the epidermis, co-stained with DAPI (nuclei, blue) and acetylated α-tubulin (Ac-α-tubulin; cilia, magenta). B) High-magnification image from the specimen shown in A. C) Katnal2 antibody staining (green) with Centrin4-CFP expression (magenta). D-H) KATNAL2-GFP (green) in the embryonic epidermis of X. laevis, co-stained for Centrin4-RFP (basal bodies/centrioles, magenta) and Ac-α-tubulin (cilia, blue). D-E) KATNAL2-GFP localizes to basal bodies in multiciliated cells. (E is close-up of D) F-H) KATNAL2-GFP localization in non-ciliated epidermal cells. G) KATNAL2-GFP signal in centrioles. H) KATNAL2-GFP signal in the midbody of dividing cells. |