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Gene/Clone | Species | Stage | Anatomy Item | Experimenter |
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gpat4 | xenopus | optic field [+] |
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Experiment details for gpat4
Identification and expression analysis of GPAT family genes during early development of Xenopus laevis.Identification and expression analysis of GPAT family genes during early development of Xenopus laevis.
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Fig. 4. mRNA expression of GPATs and DHAPAT during neurulation and early organogenesis detected by whole-mount in situ hybridization. Expression is first detected at late gastrulation (stage 12/13) and early neurulation. All the GPATs and DHAPAT are expressed in a region around the blastopore (b) and along the anterior-posterior embryonic axis, towards the dorsal midline (dm). At stage 17, GPATs and DHAPAT are expressed in the nervous system, including the neural tube (nt), hindbrain (hb), the forebrain (fb) and the eye primordia (ep). This pattern becomes more evident at stage 19. Nervous system expression is observed in dorsal and anterior views of embryos at stage 20 and older. Vax2 and ET are two genes encoding transcription factors involved in early eye development, and were used for reference. Stage 20/22 left panel, dorsal view; Stage 17, 19, 20/22 right panel and 23, anterior view. | |||||||||||||||||||||
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Fig. 5. mRNA expression of GPATs and DHAPAT in embryos and in kidney between stages 32 and 37/38 as detected by whole-mount in situ hybridization. mRNA for all GPATs and DHAPAT are present in the kidney (K), as well as in the nervous system, including the eye (E), otic vesicle (OV), spinal cord (SC) and branchial arches (BA). GPAT1 and GPAT4 are also expressed in the liver (L). Lateral views at the indicated stages (anterior to left). GPAT1, GPAT2, GPAT4, and DHAPAT are expressed in the pronephric tubules (arrows) and ducts (arrowheads), but GPAT3 is not expressed in the pronephric ducts (white arrowhead). |