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Fig. 4. In situ hybridization showing vegetally localized genes. Unfertilized eggs were obtained from albino (A–K) and wild-type (E′–
K′) Xenopus laevis. XL002d04 (A), XL003i03 (B), XL099d22 (C), and XL105l17 (VegT) (D) were expressed uniformly in the vegetal
hemisphere. XL037i04 (Xotx1) (E, E′), XL087b08 (XNIF) (F, F′), and XL081p07 (Vg1) (G, G′) were gradually expressed with the most
condensed staining at the vegetal pole. XL13k14 (I, I′), XL026p20 (Xdazl) (J, J′), and XL096g22 (Xpat) (K, K′) were expressed in the
germ plasm. XL010a22 (Xwnt11) (H, H′) was gradually expressed with the most condensed staining at the vegetal pole, and also
expressed in the germ plasm (arrows). (A–G) Lateral view of unpigmented eggs with the animal pole on the top, and (H–K) Vegetal
view. (E′–K′) Cut face of the egg, which was bisected along animal-vegetal axis, with the pigmented animal pole on the top. Lowest panels
were higher magnification view of H′–K′. Scale bar in A–K, approximately 200 μm. |
