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six3xenopus cement gland primordium [+] 

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Experiment details for six3

Murato Y and Hashimoto C (2009) Assay

Xhairy2 functions in Xenopus lens development by regulating p27(xic1) expression.

Gene Clone Species Stages Anatomy
six3 xenopus NF stage 15 cement gland primordium

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  Figure 4. Xhairy2 knockdown reduces the expression of LF marker genes in neurulae without marked changes in PPE marker expression. Micro-injection and lineage tracing were performed as described in Figure 3. Injected MO is indicated at the upper right corner and WISH probe is indicated at the lower right corner of each panel. a: Schematic presentation of LF in neurulae. b-i: LF marker genes. b, c: Two-color WISH of st.-15 embryos to confirm LF position. LF expression (black arrowheads) of pax6 (magenta) and six3 (purple) is indeed outside of the neural plate border that is marked by Xhairy2 (light blue). j, k: Embryos were injected with 6.9 ng of Xhairy2 MO and/or 20 pg of Xhairy2b mRNA where Xhairy2 MO does not bind in order to check the specificity of Xhairy2 MO to LF-loss phenotypes. I: Quantitative summary of j and k. Error bar: standard deviation of 5 independent experiments. m-t: PPE marker genes. Red arrowheads indicate reduction of marker gene expression. All the samples were pictured from the anterior side with the dorsal side up.

Gene Clone Species Stages Anatomy
six3 xenopus NF stage 15 cement gland primordium

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  Figure 7. Inhibition of cell cycle from gastrula to mid-neurula stage by HUA treatment does not affect the formation of LF and lens. a, b: BrdU detection to confirm effects of HUA. a', b': Transverse sections indicated by red lines in a, b. c: Counting of BrdU-positive cells. A representative result is shown. Error bar: standard deviation (n = 5). d-i': HUA treatment from st. 10+ to st. 15. d, e: LF expression of pax6 together with mitotic antigen PHH3. f, g: LF expression of six3. h-i': HUA-treated embryos after a wash from st. 15 showed less tail elongation (i) at st. 38 than control embryos (h). h', i': Transverse section of the embryos shown in h and i, respectively. Note that lenses were formed in HUA-treated samples (i'), although their sizes were smaller than that of control sample (h').