| |
FIG. 4. XGREUL1 is expressed in the cement gland, cranial
placodes, and the pronephros. Whole-mount in situ hybridization
of Xenopus embryos using XGREUL1 antisense and sense probes.
(A) Eight-cell-stage embryos; upper embryo showing GREUL1
expression in the animal hemisphere, the lower embryo is a sense
control. (B) Anterior view of stage 20 embryos exhibiting cement
gland staining, lower embryo is the sense control. Embryos were
cleared with benzyl benzoate:benzyl alcohol (1:1). Stage 27 (C),
stage 39 (D), and stage 42 (E) embryos expressing XGREUL1 in the
lateral line system, the pronephros, the olfactory placode, and the
otic vesicle. (F) RT-PCR showing GREUL1 expression during
Xenopus development and using ODC as a control. Abbreviations:
cg, cement gland; m, migratory primordium of middle trunk line;
pAD, anterodorsal lateral line placode; pd, pronephric duct; g,
pronephric tubules; pOl, olfactory placode; ot, otic vesicle. |
