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Fig. 4. Pattern of histone H4 acetylation and H1° accumulation in butyrate treated embryos taken at stage 27. Successive cryosecrions were
obrained from treated embryos taken at stage 27 of development and immunodetection of these sections was carried our as in Fig. 2. Here again
H4- indicates aeery/aced H4, R17shows nuclei containing hyperacetyfated H4, and H1° indicates the presence of histone H 1°.As In Fig. 2. nuclei in
each section are visualized by Hoechsr fluorescence and shown in the left side of rhe immunofluorescence (FITC) panel. Arrows are used to show
underacetylatedregions.InHIDpanel.N refers to the nervoussystem andS to the som/ces. |