| |
Figure 4. Changes in anterior neural marker expression elicited by removal of RAR-mediated repression. Whole-mount in situ hybridization analysis of Otx2 (a,c,i,k) or BF1/Xen2 (b,d,j,l). (a,b,e,f) Control embryos injected with GFP mRNA. (c,d,g,h) Embryos injected with the control morpholino oligonucleotide. (i,j) Embryos injected with 4 ng of C-SMRT and 200 pg of GFP mRNAs. (k,l) Embryos injected with 10 ng of morpholino antisense oligonucleotide directed against xRARα2 and 200 pg of GFP mRNA. (e–h,m–p) Fluorescence view of the embryos shown in a–d and i–l, revealing the location of the GFP lineage tracer. Embryos were fixed at stage 16–18 and are shown with dorsal upward. |
