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Gene/Clone	Species	Stage	Anatomy Item	Experimenter
sebox	xenopus		involuted dorsal mesoderm [+] Eberth-Kastschenko layer,abdominal myoblast,anterior notochord,archenteron roof midline,axial ossicle,caudal presomitic mesoderm,cranial skeleton,dermatome,dermomyotome,dorsal endoderm,dorsomedial edge of myotome,flexural organ,left-right organizer,left-right organizer primordium,mandibular mesenchyme,monociliated cell,myocoelic slit,myotome,notochordal sheath,perichordal tissue,postcranial axial skeleton,posterior notochord,prechordal plate,presomitic mesoderm,rib,rostral presomitic mesoderm,sclerotomal cartilage,sclerotomal mesenchyme,sclerotome,stratum compactum,stratum spongiosum,syndetome,transverse process,urostyle,vacuolated notochordal tissue,ventrolateral edge of myotome,vertebra,vertebral centrum,vertebral column,vertebral element,axial mesoderm,axial skeleton,cervical vertebra,dermatome,dermis,elastica externa,flexural organ,gastrocoel roof plate,head somite 1,head somite 2,head somite 3,head somite 4,myotome of head somite 1,myotome of head somite 2,myotome of head somite 3,myotome of head somite 4,myotome of tail somite 14,myotome of tail somite 15,myotome of tail somite 16,myotome of tail somite 17,myotome of tail somite 18,myotome of trunk somite 1,myotome of trunk somite 10,myotome of trunk somite 11,myotome of trunk somite 12,myotome of trunk somite 13,myotome of trunk somite 2,myotome of trunk somite 3,myotome of trunk somite 4,myotome of trunk somite 5,myotome of trunk somite 6,myotome of trunk somite 7,myotome of trunk somite 8,myotome of trunk somite 9,notochord,notochordal epithelium,paraxial mesoderm,presomitic mesoderm,presumptive axial mesoderm,presumptive paraxial mesoderm,sacral vertebra,sclerotome of head somite 4,sclerotome of tail somite 14,sclerotome of tail somite 15,sclerotome of tail somite 16,sclerotome of tail somite 17,sclerotome of tail somite 18,sclerotome of trunk somite 1,sclerotome of trunk somite 10,sclerotome of trunk somite 11,sclerotome of trunk somite 12,sclerotome of trunk somite 13,sclerotome of trunk somite 2,sclerotome of trunk somite 3,sclerotome of trunk somite 4,sclerotome of trunk somite 5,sclerotome of trunk somite 6,sclerotome of trunk somite 7,sclerotome of trunk somite 8,sclerotome of trunk somite 9,somite,tail somite 14,tail somite 15,tail somite 16,tail somite 17,tail somite 18,trunk somite 1,trunk somite 10,trunk somite 11,trunk somite 12,trunk somite 13,trunk somite 2,trunk somite 3,trunk somite 4,trunk somite 5,trunk somite 6,trunk somite 7,trunk somite 8,trunk somite 9,urostyle

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Experiment details for sebox

Chen G et al. (2015) Assay

Sebox regulates mesoderm formation in early amphibian embryos.

Gene	Clone	Species	Stages	Anatomy
sebox.L		laevis	NF stage 11	involuted dorsal mesoderm

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Fig. 2. Activation of sebox expression by Nodal/Activin signaling. A: WISH in mid-gastrula stage (st11) embryos injected with 80 pg Xnr1 mRNA into one cell at the four-cell stage and analyzed for sebox and brachyury (bra) expression. All embryos were viewed from the lateral side (lat.) with the animal pole upward. B: qRT-PCR analysis of the expression of sebox, bra, goosecoid (gsc) and chordin (chrd) in animal caps (ACs) in response to Activin treatment. Animal caps were dissected at st8.5 and cultured with the indicated dose of Activin for 4 hr before being subjected to RTPCR analysis. The gene expression levels in the control whole embryos (WE) were set to 100% for normalization and odc served as a loading control. C: WISH of st11 embryos injected with 2 ng cerberus-short (Cer-S) mRNA at the two-cell stage and analyzed for sebox and bix4 expression. Embryos were viewed from the vegetal (veg.) pole with the dorsal side upward. D: qRT-PCR analysis of the relative expression of the indicated genes in mid-gastrula (st11) embryos with treatments, as shown in C. The expression level of odc was used for normalization. E: RT-PCR analysis of the expression of the indicated genes in animal cap explants at the equivalent stage 11. Animal caps were isolated from st8.5 embryos that were injected with 0.5 ng VegT mRNA with or without 2 ng DN-ActRII mRNA at the two-cell stage. The marker odc served as a loading control. (F) RT-PCR analysis of the expression of the indicated genes in st11 animal cap explants that were isolated from st8.5 embryos injected with a range of doses (0–300 pg) of bra mRNA. The marker odc served as a loading control.

Gene	Clone	Species	Stages	Anatomy
sebox.L		laevis	NF stage 11	involuted dorsal mesoderm

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Fig. 3. Modulation of sebox expression by zygotic Wnt/b-catenin signaling. A: WISH for sebox expression in stage 11 embryos injected with or without 40 pg Wnt8 plasmid at the two-cell stage. B: WISH for sebox, bix4 and mixer expression in embryos with the indicated treatments. Embryos were injected with 30 ng b-catenin (b-cat) Morpholino (MO) at the two-cell stage and cultured with or without 100 mM NaCl or LiCl at stage 8.5 for 30 min. They were then cultured in fresh 0.1 MMR to stage 11, followed by WISH analysis. ND: not determined. C: WISH for sebox, bix4 and mixer expression in stage 11 embryos injected with 20 ng Wnt8 MO at the two-cell stage. Note that mixer probes were applied to both intact embryos and half embryos that were bisected along the dorsal–ventral body axis (the bottom row). D: qRT-PCR analysis of the expression of sebox and mixer in animal caps in response to Activin treatment. Animal caps from 30 ng cMO or b-cat MO-injected embryos were dissected at st8.5, cultured with Activin (2 ng/ml) for 4 hr and then subjected to RT-PCR analysis. The relative expression of the indicated genes was normalized to the level of odc. The p values were calculated by a Student’s t-test. n.s. (no significance). E: qRT-PCR analysis of the expression of sebox, bra, and gsc in animal caps with the indicated treatments. Animal caps were dissected at stage 8.5 from wild-type (ctrl) or b-catenin mRNA (150 pg)-injected embryos and cultured with or without a low dose of Activin (0.1 ng/ml) protein for 4 hr. They were then subjected to qRT-PCR analysis. The gene expression levels in whole embryos were set to 100%. The relative expression levels of genes were normalized to the levels of odc.