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Gene/Clone	Species	Stage	Anatomy Item	Experimenter
fus	xenopus		involuted dorsal mesoderm [+] Eberth-Kastschenko layer,abdominal myoblast,anterior notochord,archenteron roof midline,axial ossicle,caudal presomitic mesoderm,cranial skeleton,dermatome,dermomyotome,dorsal endoderm,dorsomedial edge of myotome,flexural organ,left-right organizer,left-right organizer primordium,mandibular mesenchyme,monociliated cell,myocoelic slit,myotome,notochordal sheath,perichordal tissue,postcranial axial skeleton,posterior notochord,prechordal plate,presomitic mesoderm,rib,rostral presomitic mesoderm,sclerotomal cartilage,sclerotomal mesenchyme,sclerotome,stratum compactum,stratum spongiosum,syndetome,transverse process,urostyle,vacuolated notochordal tissue,ventrolateral edge of myotome,vertebra,vertebral centrum,vertebral column,vertebral element,axial mesoderm,axial skeleton,cervical vertebra,dermatome,dermis,elastica externa,flexural organ,gastrocoel roof plate,head somite 1,head somite 2,head somite 3,head somite 4,myotome of head somite 1,myotome of head somite 2,myotome of head somite 3,myotome of head somite 4,myotome of tail somite 14,myotome of tail somite 15,myotome of tail somite 16,myotome of tail somite 17,myotome of tail somite 18,myotome of trunk somite 1,myotome of trunk somite 10,myotome of trunk somite 11,myotome of trunk somite 12,myotome of trunk somite 13,myotome of trunk somite 2,myotome of trunk somite 3,myotome of trunk somite 4,myotome of trunk somite 5,myotome of trunk somite 6,myotome of trunk somite 7,myotome of trunk somite 8,myotome of trunk somite 9,notochord,notochordal epithelium,paraxial mesoderm,presomitic mesoderm,presumptive axial mesoderm,presumptive paraxial mesoderm,sacral vertebra,sclerotome of head somite 4,sclerotome of tail somite 14,sclerotome of tail somite 15,sclerotome of tail somite 16,sclerotome of tail somite 17,sclerotome of tail somite 18,sclerotome of trunk somite 1,sclerotome of trunk somite 10,sclerotome of trunk somite 11,sclerotome of trunk somite 12,sclerotome of trunk somite 13,sclerotome of trunk somite 2,sclerotome of trunk somite 3,sclerotome of trunk somite 4,sclerotome of trunk somite 5,sclerotome of trunk somite 6,sclerotome of trunk somite 7,sclerotome of trunk somite 8,sclerotome of trunk somite 9,somite,tail somite 14,tail somite 15,tail somite 16,tail somite 17,tail somite 18,trunk somite 1,trunk somite 10,trunk somite 11,trunk somite 12,trunk somite 13,trunk somite 2,trunk somite 3,trunk somite 4,trunk somite 5,trunk somite 6,trunk somite 7,trunk somite 8,trunk somite 9,urostyle

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Experiment details for fus

Dichmann DS and Harland RM (2012) Assay

fus/TLS orchestrates splicing of developmental regulators during gastrulation.

Gene	Clone	Species	Stages	Anatomy
fus.S		laevis	NF stage 11	involuted dorsal mesoderm
fus.S		laevis	NF stage 11.5	involuted dorsal mesoderm

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Figure 1. fus is expressed in the animal region, and knockdown results in gastrulation defects and animal dissociation. All pictures show a lateral view with the animal region up, except for D and H, which show vegetal view. (A–C) Normal embryos bisected and subjected to ISH with fus antisense probe. (A) Initial fus staining at stage 9 is confined to the animal region. (B) At mid-gastrulation, fus is expressed strongly throughout the prospective mesodermal and ectodermal regions but is excluded from the endoderm. (C) This pattern persists through gastrulation, although some weak staining can be observed in vegetal cells. (D–K) fus knockdown results in lack of blastopore formation and cell dissociation. (D,E) Normal embryos at mid-gastrulation, with prospective mesendoderm involuting at the blastopore. (H,I) fus morphants show complete lack of blastopore formation and gastrulation movements. (F) Normal embryo showing intact epithelia. (J) Individual cells detach from the animal and marginal regions in fus morphants. (G,K) Magnified view of the yellow box in F and J, respectively. (L–O) Absence of apoptosis during gastrulation in fus morphants. Control (L,M) and fusMO (N,O) embryos TUNEL-stained for apoptotic cells at cell dissociation (L,N) or after cell dissociation occurs (M,O). No apoptotic cells were detected at stage 12, when cell dissociation happens. At stage 13, some apoptotic cells that were detected in fus have dissociated and therefore mostly likely show a secondary effect. (P) Single-embryo RT–PCR spanning the first four exons of fus on embryos injected with fusMO4 showing aberrant splicing of the first intron in fus transcripts. Normal transcripts of both homeologs (fus-a and fus-b) in X. laevis are significantly reduced relative to control odc transcripts.

Gene	Clone	Species	Stages	Anatomy
fus.S		laevis	NF stage 11	involuted dorsal mesoderm

fus (fused in sarcoma) gene expression in bisected Xenopus laevis embryo, mid-sagittal section, assayed via in situ hybridization, NF stage 11, dorsal right, anterior up.

Gene	Clone	Species	Stages	Anatomy
fus.S		laevis	NF stage 11.5	involuted dorsal mesoderm

fus (fused in sarcoma) gene expression in bisected Xenopus laevis embryo, mid-sagittal section, assayed via in situ hybridization, NF stage 11.5, dorsal right, anterior up.