|
Figure S2. FGF signaling is not required for maintaining foregut progenitors, but is required for lung and liver induction in a dose-dependent manner. (A) Embryos cultured in DMSO and FGFRi from stages NF15 to NF23 or injected with RNA encoding β-gal (3 ng) or dnFGFR (3 ng) were analyzed for expression of the foregut progenitor marker hhex and the cardiac progenitor marker nkx2.5. The graph summarizes the percentage of embryos with normal, weak or severely reduced/absent expression. (B) Embryos cultured from stages NF18 to NF35 in DMSO or FGFRi at the indicated concentrations and the percent of embryos with normal, weak, or severely reduced/absent nr1h5 and nkx2.1 expression was scored at stage NF35. |