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FIG. 3. Temporal and spatial expression of Ximpact. (A) Expression
of Ximpact during embryogenesis. Total RNAs (25 ng) isolated
from embryos at different stages (stages 3, 6, 9, 10, 13, 19, 26, and 33)
were used for RT-PCR assay. (B) Spatial distribution of Ximpact
transcript in embryonic tissues. Embryos at 8-cell stage were dissected
into four portions, namely, animal-dorsal (AD), vegetal-dorsal
(VD), animal-ventral (AV), and vegital-ventral (VV) regions as
shown in the upper left panel. Total RNAs isolated from these portions
were subjected to the RT-PCR assay (upper right panel). Embryos
at the neurula stage were dissected into notochord, dorsal
ectoderm, dorsal endoderm, somite, and belly (lower left), and RNA
from each portion was examined by the RT-PCR assay (lower right).
(C) Distribution of Ximpact transcript in adult tissues. The RNAs
from adult brain, skin, heart; stomach, liver; kidney, lung, intestine,
testis, ovary, and muscle were examined by RT-PCR. |
