Search Criteria |
Gene/Clone | Species | Stage | Anatomy Item | Experimenter |
---|---|---|---|---|
tent5a | xenopus | optic vesicle [+] |
Too many results?Too few results?
Experiment details for tent5a
Fam46a regulates BMP-dependent pre-placodal ectoderm differentiation in Xenopus.Fam46a regulates BMP-dependent pre-placodal ectoderm differentiation in Xenopus.
|
Display additional annotations [+] |
|||||||||||||||
Fig. 1. Temporal and spatial expression pattern of Fam46a [tent5a] in Xenopus. (A) RT-PCR analysis of Fam46a expression. The expression of Fam46a is faintly detected from the maternal stage to stage 9, and increased from stage 10, similar to that of Zic1 (NPB), Vent1, Vent2 (BMP target gene), and Chordin (BMP antagonist). The expression of PPE marker genes, Six1 and Eya1, increased soon after the onset of Fam46a expression. ODC was used as a control. (B-E) Whole-mount in situ hybridization (WISH) was performed with a Fam46a (B, B’) and Six1 (C, C’) probe. The anterior view (B, C); the lateral view (D, E); the hemi-section (B’, C’, D’, D”, E’). Development • Accepted manuscript The stage of the embryo was shown in each panel. PPE, pre-placodal ectoderm; Op, optic vesicle; Ot, otic vesicle; Le, lens; Br, branchial arch mesenchyme; RPE, retinal pigment epithelium. (F) HeLa cells were transfected with GFP-Fam46a and cultured for 24 hours. Nuclei were stained with NUCLEAR-ID Red. Scale bars represent 20 μ m. | ||||||||||||||||
|
|
|||||||||||||||
tent5a (terminal nucleotidyltransferase 5A) gene expression in Xenopus laevis, assayed by in situ hybridization, NF stage 28, transverse section, dorsal up. | ||||||||||||||||
|
|
|||||||||||||||
tent5a (terminal nucleotidyltransferase 5A) gene expression in Xenopus laevis, assayed by in situ hybridization, NF stage 28, anterior left. |