Search Criteria |
Gene/Clone | Species | Stage | Anatomy Item | Experimenter |
---|---|---|---|---|
dyrk1a | xenopus | optic vesicle [+] |
Too many results?Too few results?
Experiment details for dyrk1a
Willsey HR et al. (2020) AssayThe neurodevelopmental disorder risk gene DYRK1A is required for ciliogenesis and control of brain size in Xenopus embryos.
|
Display additional annotations [+] |
|||||||||||||||||||||||||
Fig. 1. Expression and localization of Dyrk1a during X. tropicalis development. (A-F) RNA in situ hybridization in X. tropicalis. (A-B′) dyrk1a is expressed during gastrulation (stage 10.5, A) and at stage 20 (B) in the brain, spinal cord, optic vesicles and ciliated epidermis (B′). (C,D) At stages 32 (C) and 35 (D), dyrk1a is expressed in the brain, spinal cord, otic vesicles, optic vesicles, pharyngeal arches, heart, epidermis and pronephros. (E,F) At stages 40 (E) and 46 (F), dyrk1a is expressed in the brain, especially along ventricles [telencephalon (tel), diencephalon (di), mesencephalon (mes) and rhombencephalon (rhomb)]. Scale bars: 500 µm; 100 µm in B′. (G-G‴) Embryonic epidermis antibody staining for Dyrk1a protein (green) shows puncta along ciliary axonemes labeled for acetylated α-Tubulin (Ac-α-Tubulin, magenta). See Fig. S1 for antibody validation. (H) Dorsal view of whole-mount antibody staining showing Dyrk1a (green) throughout the nervous system and in the pronephros (pro). (I-J′) Dyrk1a (green) is on cilia marked by polyglutamylated-Tubulin (polyglut-Tubulin, magenta) in the olfactory epithelium (olf, I,I′) and in the rhombencephalon (J-J′). White arrows (I′) indicate the puncta of Dyrk1a (green) on cilia. There is also strong membrane staining. (K-K′) Human GFP-tagged DYRK1A (hDYRK1A-GFP, green) localizes in puncta along ciliary axonemes labeled for acetylated α-Tubulin (magenta). (L-L′) hDYRK1A-GFP (green) colocalizes with Centrin-CFP (cyan) at basal bodies. | ||||||||||||||||||||||||||
|
|
|||||||||||||||||||||||||
dyrk1a (dual specificity tyrosine-(Y)-phosphorylation regulated kinase 1A) gene expression in a Xenopus tropicalis embryo, assayed via in situ hybridization, NF stage 20, lateral view, anterior left, dorsal up. | ||||||||||||||||||||||||||
|
|
|||||||||||||||||||||||||
dyrk1a (dual specificity tyrosine-(Y)-phosphorylation regulated kinase 1A) gene expression in a Xenopus tropicalis embryo, assayed via in situ hybridization, NF stage 35 & 36, lateral view, anterior left, dorsal up. | ||||||||||||||||||||||||||
|
|
|||||||||||||||||||||||||
dyrk1a (dual specificity tyrosine-(Y)-phosphorylation regulated kinase 1A) gene expression in a Xenopus tropicalis embryo, assayed via in situ hybridization, NF stage 40, dorsal view, anterior up. | ||||||||||||||||||||||||||
|
|
|||||||||||||||||||||||||
Whole-mount antibody Dyrk1a Ab1 [AB_1209558] staining showing Dyrk1a (green) throughout the nervous system and in the pronephros in a stage NF stage 46, Xenopus tropicalis embryo, dorsal view, anterior up. Key: olf: olfactory epithelia, tel: telencephalon, di: diencephalon, mes: mesencephalon/midbrain, rhomb: rhombencephalon/hindbrain, pro: pronephric kidney, eye: eye. |