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Fig. 2. Differential distribution of presynaptic proteins in the glomerular layer of the MOB. Horizontal sections of the MOB labelled with antibodies to synaptophysin (A), syntaxin (B) and SNAP-25
(C). All three antibodies label glomeruli in all glomerular clusters (int, intermediate glomerular cluster; lat, lateral glomerular cluster; med, medial glomerular cluster). The axes in (A) indicate: A, anterior; P,
posterior; L, lateral; M, medial. (D) Horizontal section of the MOB anterogradely labelled with biocytin through the olfactory nerve. Double-staining of the olfactory receptor axons with biocytin in (D) clearly
visualizes three glomerular clusters. (E) Synaptotagmin immunostaining of the same slice shown in (D). Synaptotagmin-immunoreactivity (IR) was limited to the lateral and partly to the intermediate glomerular
cluster. (F) An overlay of the two channels (D and E) accents the unequal distribution of synaptotagmin-IR in the different clusters. Scale bars: 100 lm. |
