|
Fig. 4. In situ hybridization of coronal sections through the sensory
epithelium of the medial and lateral diverticulum of Xenopus
using Ga subtype-specific antisense RNA probes. Coronal sections
were hybridized with DIG-labeled antisense RNA specific for Gasx2
(A,B) and Gaox1 (C,D). Hybridization with the Gasx2-specific probe
intensely labeled the olfactory sensory cell layer (A), whereas hybridization
signals in the lateral diverticulum are low (B). Hybridization
with the Gaox1-specific probe led to very few hybridization signals in
the medial diverticulum (C), whereas many sensory cells in the lateral
diverticulum were labeled (D). oe, olfactory neuron layer; sc,
sustentacular cells; bs, basal cells; lp, lamina propria; bg, Bowman‘s
glands. Scale bar 5 20 mm. |