|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
hoxa13
|
|
laevis
|
NF stage 52
|
forelimb
,
forelimb bud
,
limb bud
,
distal mesenchyme
|
hoxd13
|
|
laevis
|
NF stage 52
|
posterior
,
hindlimb bud
,
limb bud
,
distal mesenchyme
|
hoxd13
|
|
laevis
|
NF stage 54
|
hindlimb
,
mesenchyme
,
autopod
,
interdigital mesenchyme
|
hoxd11
|
|
laevis
|
NF stage 52
|
posterior
,
hindlimb bud
,
limb bud
,
distal mesenchyme
|
hoxd11
|
|
laevis
|
NF stage 54
|
hindlimb
,
limb bud
,
autopod
,
interdigital mesenchyme
|
tbx3
|
|
laevis
|
NF stage 52
|
lateral
,
mesenchyme
,
anterior
,
posterior
,
hindlimb bud
,
[+]
|
tbx3
|
|
laevis
|
NF stage 54
|
hindlimb
,
mesenchyme
,
anterior
,
posterior
,
hindlimb bud
,
[+]
|
sox9
|
|
laevis
|
NF stage 52
|
hindlimb bud
,
limb bud
,
cartilage element
|
sox9
|
|
laevis
|
NF stage 54
|
hindlimb
,
hindlimb digit
,
limb bud
,
cartilage element
|
|
|
Figure 2. Gene expression in the Xenopus developing hindlimb. A-E: Gene expression in the forelimb bud at the paddle stage (Nieuwkoop and Faber stage 52). A:Hoxa13 expression. Digit cartilage started to differentiate and hoxa13 expression was downregulated (compare with 3E). B:hoxd13 expression. In the anterior domain, weak but clear expression of hoxd13 was detected. C:Hoxd11 expression. The expression pattern was similar to the pattern in the forelimb (compare with 2C, 3C). D:Tbx3 expression. Expression of tbx3 was present in both the anterior and posterior margins.E:Sox9 expression. The first differentiating digit cartilage was observed (arrow). T; tibia (tibiale). F; fibula (fibulare). F-J: Gene expression in the forelimb bud at the digit stage (Nieuwkoop and Faber stage 54). F:Hoxa13 expression. Hoxa13 expression remained throughout the autopodium.G:hoxd13 expression. In the anterior domain, weak but clear expression of hoxd13 was still detectable (arrows in G,G'). H:Hoxd11 expression. Arrows in H and H' indicate the expression border. I:Tbx3 expression. Arrows in I indicate the expression border. J:Sox9 expression. The cartilage of putative digit I was just starting to express sox9 (arrow in J'). F'-J': Higher magnification of each figure. II-V are the digit numbers suggested by our results. Distal is to the top, and anterior is at the left. |