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Fig. 8: ANKS1A function is highly conserved in Xenopus. a 3DSIM images were obtained from the epidermis of Xenopus embryos at stage 24. The BB patch outlined with white dotted lines was magnified for further analysis. A representative Fop image at ×7 magnification of the region indicated by the yellow box. The central angle of the Fop-negative region is clearly visible. b The data in a were quantified. The data represent mean ± SD. Each point on the graph represents the central angle of a Fop-negative region. DMSO injection, n = 501 BBs from 3 embryos; anks1a-MO injection, n = 645 BBs from 3 embryos. c, d Experiments were performed essentially as described in a, b, except that the 3DSIM images were obtained from the epidermis of Xenopus embryos at stage 35. DMSO injection, n = 905 BBs from 5 embryos; anks1a-MO injection, n = 854 BBs from 5 embryos. anks1a-MO plus mouse ANKS1A-VN RNA injection, n = 726 BBs from 3 embryos. e Experiments were performed as described in Fig. 4b. Scale bar for a, c, e, 2 μm. f Model in which ANKS1A plays a pivotal role in the polarization of SDAs during the development of MCCs. In the absence of ANKS1A, the SDA BF adopts an unstable architecture and undergoes a gradual degeneration over the course of aging, causing the loss of the BBs and motile cilia. |
